The technique of genetic transduction has been applied to the exchange of flagellar antigens among various serotypes of Salmonella, somatic groups B and D. Each transduction resulted in the substitution or transfer of a single antigenic phase, whether to or from a monophasic or diphasic recipient strain. The types generated include several serotypes previously discovered and named, and others which so far have no place in the Kauffmann-White scheme. However, the precise role of transduction in the natural evolution of serotypes must be settled by further studies.
In the present material, the transduction of a flagellar antigen was unaccompanied by any other alterations in antigenic structure or biochemical behavior, so far as studied. In a few instances, transduction revealed the character of antigenic determinants which were not directly expressed in the source strain. Thus, the presence of an a phase in S. abortus-equi (IV XII [a]:enx) was confirmed, while S. gallinarum proved to carry a determinant for the gm antigen, which is unexpressed in the absence of flagella. In no case were the component factors of the complex antigens of a single phase (viz. 1,2…, e,h…, g,p…, e,n,x…, 1,5…) separated in transduction, and such tests as were made indicated that the transduced antigen was identical with the phase of the source strain.
Department of Genetics Paper No. 518. The work at Madison was supported by research grants (E72-C3) from the National Microbiological Institute of the National Institutes of Health, Public Health Service and from the Research Committee of the Graduate School, University of Wisconsin, with funds provided by the Wisconsin Alumni Research Foundation.