1. Ca++ is required in the formation of the complex EAC′x.

  2. The combination of EA with C′x is a reaction which essentially goes to completion.

  3. The velocity of formation of EAC′x is greater at 34°C than at 0°C. However, a more reactive EAC′x is obtained at 0°C.

  4. The reaction of EAC′x with C′y requires Mg++.

  5. The velocity and final extent of reaction of EAC′x with C′y, depends on the concentration of C′y, as well as on the concentration of C′x per cell.

1

The present work was supported by grants from the National Science Foundation, Cone Foundation and by a contract (121–104) with the Office of Naval Research.

2

A preliminary report of the present work was presented at the 1954 meeting of the Federated Societies for Experimental Biology. (Federation Proceedings, Vol. 13, page 504, 1954.)

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