The use of both I131 labelled antigens and I131 labelled antibodies allowed calculations of the antigen-antibody ratios of the soluble complexes precipitated by complement in the different regions of antigen excess. The antigen-antibody molecular ratios of the “limiting” complexes, precipitated in extreme antigen excess, afforded a calculation of the valence of the antibody. A valence of approximately 2 was obtained for rabbit anti-BSA and anti-BGG. These values were supported by determinations made with soluble complexes precipitated with chicken anti-RGG.

1

Presented at the 55th annual meeting of the Society of American Bacteriologists, May, 1955, New York, N. Y. Presented by William O. Weigle in partial fulfillment of the requirements of the degree of Doctor of Philosophy, University of Pittsburgh, Pittsburgh, Pennsylvania. Supported in part by U. S. Atomic Energy Commission Contract No. AT(30-1)-1205.

2

Publication No. 97 of the Department of Pathology, University of Pittsburgh School of Medicine.

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