Pooled serums from normal, adult, male Sprague-Dawley rats have been analyzed by the electrophoretic methods of moving boundary, filter paper, polyvinylchloride block, starch gel, immunoelectrophoresis and starch gel immunoelectrophoresis. Immunoelectrophoresis has been found to be the most sensitive single procedure. Twenty-five antigenic components of rat serum were observed by this technique and a standard system of nomenclature was proposed. The antigenic heterogeneity of fractions separated by other methods was demonstrated.

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This study was supported in part by research grants from the National Cancer Institute (C-2368) and from the California Institute for Cancer Research.

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Copyright, 1963, by The Williams & Wilkins Company
Copyright © 1963 by The American Association of Immunologists, Inc.
1963
The Williams & Wilkins Company