A highly allergenic protein-carbohydrate preparation has been isolated from a low ragweed pollen extract (Ambrosia elatoir) by precipitation with ammonium sulfate followed by chromatography on DEAE-cellulose, gel-filtration on Sephadex G-75, chromatography on DEAE-Sephadex and gel-filtration on Sephadex G-200. The preparation is skin reactive at 10-9 to 10-10 µg, on intradermal injection, in the treated rag-weed-sensitive patient. Immunoelectrophoretic and gel-diffusion methods show multiple antigens.

1

This investigation was supported in part by a Grant (AI 03185) from the National Institute of Allergy and Infectious Diseases.

This content is only available via PDF.
Copyright, 1963, by The Williams & Wilkins Company
Copyright © 1963 by The American Association of Immunologists, Inc.
1963
The Williams & Wilkins Company