The electrophoretic patterns in starch gel in 8 M urea of the L chains of dissociated guinea pig and rat anti-DNP antibodies were investigated. Guinea pig anti-DNP antibodies isolated from weekly bleedings of individual animals or from pooled guinea pig antisera yielded 5 L chain bands in urea starch gel. Rat anti-DNP antibodies also showed bands in the zone of L chain migration, but their pattern differed from that of guinea pig antibodies.

Guinea pig γ1 and γ2 anti-DNP antibodies yielded the same 5 L chain bands.

A study of the fractions of guinea pig or rat anti-DNP antibodies separated by zone electrophoresis at pH 8.6 revealed that different L chains are obtained from antibodies with different electrophoretic mobilities.

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This work was supported by the United States Public Health Service, Grant AI-04983, and by the Health Research Council of the City of New York under contract No. I-138.

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