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Flow cytometric analysis of lymphocyte subsets in rhesus macaque peripheral...
Published: 17 July 2024
FIGURE 1. Flow cytometric analysis of lymphocyte subsets in rhesus macaque peripheral blood using 15-marker Ab panel. Data shown are from NHP2 at baseline prior to any experimental procedures. The gating scheme shown was used to identify lymphocytes (17.5% of total PBMCs) via their forward scatter and side scatter. T cells were identified as CD3+ and made up 52.4% of CD45+ cells. Of the CD3+ T cells, 69.5% were CD4+ and 23.7% were CD8+. Of the CD45+ cells, B cells were defined as CD20+ (38.3%), and NK and NKT cells appear in the CD159a+ population (6.55%), which can be further subdivided (not shown) as CD3 for NK cells and CD3+ for NKT cells. More about this image found in Flow cytometric analysis of lymphocyte subsets in rhesus macaque peripheral...
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Measurement of naive cells and putative RTEs in CD4<sup>+</sup> peripheral ...
Published: 17 July 2024
FIGURE 2. Measurement of naive cells and putative RTEs in CD4+ peripheral blood T cells in rhesus macaques using 15-marker Ab panel. Data shown are from NHP2 at baseline prior to any experimental procedures. CD4+ cells were gated on CD45+CD3+CD4+ cells as shown in Fig. 1 . The gating schemes used to identify naive CD4+ cells (CD28+, CD95) versus (CD45RA+, CD197/CCR7+) are shown. Naive cells were assessed for CD31+ and CD197+ that have been reported to identify recent thymic emigrants (RTEs) in human CD4+ T cells. The percentages of double-positive cells are 99.3 or 96.8% depending on the naive gating scheme used. The exploratory marker of PTK7 was assessed on naive CD4+ cells in combination with CD31. The percentages of double-positive cells were 0.007 or 0.14% depending on the naive gating scheme used. More about this image found in Measurement of naive cells and putative RTEs in CD4+ peripheral ...
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Measurement of naive cells and putative RTEs in CD8<sup>+</sup> peripheral ...
Published: 17 July 2024
FIGURE 3. Measurement of naive cells and putative RTEs in CD8+ peripheral blood T cells in rhesus macaques using 15-color Ab panel. Data shown are from NHP2 at baseline prior to any experimental procedures. CD8+ cells were gated on CD45+CD3+CD8+ cells as shown in Fig. 1 . The gating schemes used to identify naive CD8+ cells (CD28+, CD95) versus (CD45RA+, CD197/CCR7+) are shown. Naive cells were assessed for CD103/integrin αEβ7+ and CD197+ that have been reported to identify RTEs in human CD8+ T cells. The percentages of double-positive cells were 0.71 or 1.20% depending on the naive gating scheme used. The exploratory marker of PTK7 is assessed on naive CD8+ cells in combination with CD103. The percentages of double-positive cells were 0.07 or 0.24% depending on the gating scheme used. More about this image found in Measurement of naive cells and putative RTEs in CD8+ peripheral ...
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Selected PBL subsets in NHPs 1–3 as a function of time after thymectomy and...
Published: 17 July 2024
FIGURE 4. Selected PBL subsets in NHPs 1–3 as a function of time after thymectomy and ATG treatment. Thymectomy was performed on day 0 for NHP1 and NHP2. NHP1 received ATG for 5 d starting on days 0 and 34. NHP2 received ATG for 5 d starting on day 0 only. ( A ) Total CD45+CD3+ cells. ( B ) Naive CD8+ cells were defined by the phenotype: CD45+CD3+CD8+ CD28+CD95. ( C ) CD8+ RTE cells were defined by the phenotype: CD45+CD3+CD8+CD28+ CD95CD103+CD197+. The distributions for the nonthymectomized animals are shown as the individual values plus the range, quartiles, and medians as box plots. More about this image found in Selected PBL subsets in NHPs 1–3 as a function of time after thymectomy and...
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Immunohistochemical reactivity of FFPE rhesus thymus with Abs that indicate...
Published: 17 July 2024
FIGURE 5. Immunohistochemical reactivity of FFPE rhesus thymus with Abs that indicate the functional status of the thymus. ( A ) Pan-CK polyclonal Ab reacts with the thymic epithelial cell (TEC) network in both the cortex (c) and medulla (m). Hassall bodies (arrows) are strongly cytokeratin-pos... More about this image found in Immunohistochemical reactivity of FFPE rhesus thymus with Abs that indicate...
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Expression of genes relevant to thymus function in biopsied rhesus thymus t...
Published: 17 July 2024
FIGURE 6. Expression of genes relevant to thymus function in biopsied rhesus thymus tissue. Normalized expression obtained using the QuantiGene assay is shown, with expression of ATP6V1A in each sample set equal to 1.0. Error bars represent the SD for the thymus tissues (Thy; n = 7). No error bars are shown for results from the single control samples of liver (Liv) and lymph node (LN). More about this image found in Expression of genes relevant to thymus function in biopsied rhesus thymus t...
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