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Table I.

Comparison of FOXP3+ DN thymocytes with other FOXP3+ thymocytes

Percentage FOXP3+ (Range)FOXP3 IntensityaPercentage CD25+ of FOXP3+ CellsPercentage TCR+ of FOXP3+ CellsPercentage CTLA-4+ of FOXP3+ CellsbPercentage CD127+ of FOXP3+ Cells
All thymocytes 1.9 ± 1.3 (0.3–44)c 56 ± 16 55.1 ± 6.8 94.5 ± 6.2 72.6 ± 9.9 26.8 ± 14.4 
CD4 SP cells 8.6 ± 2.5 (4.6–13.2) 59 ± 17 61.2 ± 7.3 99.0 ± 1.4 73.5 ± 13.1 17.6 ± 7.6 
DN cells 1.4 ± 1.1 (0.1–3.8) 58 ± 21 22.3 ± 6.4 32.3 ± 24.1 61.9 ± 14.4 24.8 ± 11.0 
Percentage FOXP3+ (Range)FOXP3 IntensityaPercentage CD25+ of FOXP3+ CellsPercentage TCR+ of FOXP3+ CellsPercentage CTLA-4+ of FOXP3+ CellsbPercentage CD127+ of FOXP3+ Cells
All thymocytes 1.9 ± 1.3 (0.3–44)c 56 ± 16 55.1 ± 6.8 94.5 ± 6.2 72.6 ± 9.9 26.8 ± 14.4 
CD4 SP cells 8.6 ± 2.5 (4.6–13.2) 59 ± 17 61.2 ± 7.3 99.0 ± 1.4 73.5 ± 13.1 17.6 ± 7.6 
DN cells 1.4 ± 1.1 (0.1–3.8) 58 ± 21 22.3 ± 6.4 32.3 ± 24.1 61.9 ± 14.4 24.8 ± 11.0 
a

Mean fluorescence intensity of FOXP3+ cells.

b

CTLA-4 staining was done after permeabilization.

c

Values are shown as mean ± SD. The values for FOXP3 and CD25 represent eight samples; for other markers n = 6.

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