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Table II.

Cytokine production by MLN and spleen cells from mice infected with H. polygyrus with and without concomitant treatment with rIL-12a

StimulusSource of CellsCytokine Production (ng/ml ± SEM)
IFN-γIL-5IL-10IL-4
 MLN     
Con A Noninfected 5.01 ± 0.09 0.305 ± 0.0 0.08 ± 0.01 0.42 ± 0.09 
 Infected 0.94 ± 0.01 24.39 ± 0.27 27.21 ± 0.09 19.86 ± 0.26 
 Infected+ rIL-12 17.33 ± 0.02 3.63 ± 0.07 5.23 ± 0.05 1.93 ± 0.06 
Anti-CD3 Noninfected 11.45 ± 0.14 1.06 ± 0.16 0.59 ± 0.10 0.45 ± 0.02 
 Infected 16.26 ± 0.17 87.33 ± 0.99 31.74 ± 0.17 41.9 ± 0.28 
 Infected+ rIL-12 18.8 ± 0.20 21.36 ± 0.12 12.78 ± 0.18 11.85 ± 0.32 
 Spleen     
Con A Noninfected 12.59 ± 0.16 0.71 ± 0.08 1.22 ± 0.03 0.33 ± 0.04 
 Infected 6.96 ± 0.03 6.81 ± 0.12 4.39 ± 0.07 3.00 ± 0.06 
 Infected+ rIL-12 13.2 ± 0.06 2.29 ± 0.09 2.04 ± 0.01 0.48 ± 0.05 
Anti-CD3 Noninfected 16.51 ± 0.08 1.0 ± 0.07 4.76 ± 0.08 0.58 ± 0.20 
 Infected 12.51 ± 0.16 14.96 ± 0.18 6.28 ± 0.19 13.77 ± 0.24 
 Infected+ rIL-12 15.26 ± 0.19 8.45 ± 0.15 2.49 ± 0.09 5.56 ± 0.16 
StimulusSource of CellsCytokine Production (ng/ml ± SEM)
IFN-γIL-5IL-10IL-4
 MLN     
Con A Noninfected 5.01 ± 0.09 0.305 ± 0.0 0.08 ± 0.01 0.42 ± 0.09 
 Infected 0.94 ± 0.01 24.39 ± 0.27 27.21 ± 0.09 19.86 ± 0.26 
 Infected+ rIL-12 17.33 ± 0.02 3.63 ± 0.07 5.23 ± 0.05 1.93 ± 0.06 
Anti-CD3 Noninfected 11.45 ± 0.14 1.06 ± 0.16 0.59 ± 0.10 0.45 ± 0.02 
 Infected 16.26 ± 0.17 87.33 ± 0.99 31.74 ± 0.17 41.9 ± 0.28 
 Infected+ rIL-12 18.8 ± 0.20 21.36 ± 0.12 12.78 ± 0.18 11.85 ± 0.32 
 Spleen     
Con A Noninfected 12.59 ± 0.16 0.71 ± 0.08 1.22 ± 0.03 0.33 ± 0.04 
 Infected 6.96 ± 0.03 6.81 ± 0.12 4.39 ± 0.07 3.00 ± 0.06 
 Infected+ rIL-12 13.2 ± 0.06 2.29 ± 0.09 2.04 ± 0.01 0.48 ± 0.05 
Anti-CD3 Noninfected 16.51 ± 0.08 1.0 ± 0.07 4.76 ± 0.08 0.58 ± 0.20 
 Infected 12.51 ± 0.16 14.96 ± 0.18 6.28 ± 0.19 13.77 ± 0.24 
 Infected+ rIL-12 15.26 ± 0.19 8.45 ± 0.15 2.49 ± 0.09 5.56 ± 0.16 
a

MLN or spleen cells pooled from three mice/group were collected at 8 days p.i. and cultured at 5 × 106/ml with 5 μg/ml of Con A or on plates coated with anti-CD3 (10 μg/ml). Cytokine production in 48-h culture supernatants was measured by ELISA. The results are displayed as the mean ± SEM of triplicate wells.

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