P2z receptor ligation promotes 86Rb+ release from macrophages
| Stimulus . | 86Rb+ (% Released)a . |
|---|---|
| None | 10b |
| 40 μM ATP | 17.5 |
| 100 μM ATP | 21 |
| 1 mM ATP | 81 |
| 5 mM ATP | 94 |
| 200 μM benzoyl benzoic-ATP | 46 |
| 1 mM benzoyl benzoic-ATP | 95 |
| 5 mM UTP | 21 |
| Stimulus . | 86Rb+ (% Released)a . |
|---|---|
| None | 10b |
| 40 μM ATP | 17.5 |
| 100 μM ATP | 21 |
| 1 mM ATP | 81 |
| 5 mM ATP | 94 |
| 200 μM benzoyl benzoic-ATP | 46 |
| 1 mM benzoyl benzoic-ATP | 95 |
| 5 mM UTP | 21 |
LPS-activated, 86Rb+-loaded macrophages were incubated for 5 min in the presence of the indicated stimulus. At the conclusion of this incubation, cells and media were separated, and the percentage of the radioactive isotope recovered in the medium was determined.
The value for the control (none) is a mean of eight individual wells; other values are averages of duplicate wells.