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Table II.

Effect of GM-CSF on the production of MIP-1α by neutrophils in response to EBVa

Cell-Associated MIP-1α (pg/ml)Secreted MIP-1α (pg/ml)Total MIP-1α (pg/ml)
Unstimulated 2 ± 1 3 ± 1 5 ± 1 
GM-CSF 3 ± 2 42 ± 14 45 ± 14 
EBV 40 ± 8* 867 ± 65** 907 ± 73** 
GM-CSF + EBV 31 ± 4 1944 ± 273* 1975 ± 274* 
Cell-Associated MIP-1α (pg/ml)Secreted MIP-1α (pg/ml)Total MIP-1α (pg/ml)
Unstimulated 2 ± 1 3 ± 1 5 ± 1 
GM-CSF 3 ± 2 42 ± 14 45 ± 14 
EBV 40 ± 8* 867 ± 65** 907 ± 73** 
GM-CSF + EBV 31 ± 4 1944 ± 273* 1975 ± 274* 
a

Neutrophils (107/ml) were preincubated with or without 1 nM GM-CSF for 1 h at 37°C and further incubated with or without EBV. Cell-associated and secreted MIP-1α were assessed by a specific ELISA. Results are expressed as pg/ml and are the mean ± SEM of four separate experiments. Values obtained for neutrophils incubated with GM-CSF or EBV alone were compared with those for unstimulated cells, and values for GM-CSF-treated neutrophils stimulated with EBV were compared with the sum of the values obtained when neutrophils were incubated with GM-CSF or EBV alone. *p <0.05, **p <0.01.

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