Effect of GM-CSF on the production of MIP-1α by neutrophils in response to EBVa
| . | Cell-Associated MIP-1α (pg/ml) . | Secreted MIP-1α (pg/ml) . | Total MIP-1α (pg/ml) . |
|---|---|---|---|
| Unstimulated | 2 ± 1 | 3 ± 1 | 5 ± 1 |
| GM-CSF | 3 ± 2 | 42 ± 14 | 45 ± 14 |
| EBV | 40 ± 8* | 867 ± 65** | 907 ± 73** |
| GM-CSF + EBV | 31 ± 4 | 1944 ± 273* | 1975 ± 274* |
| . | Cell-Associated MIP-1α (pg/ml) . | Secreted MIP-1α (pg/ml) . | Total MIP-1α (pg/ml) . |
|---|---|---|---|
| Unstimulated | 2 ± 1 | 3 ± 1 | 5 ± 1 |
| GM-CSF | 3 ± 2 | 42 ± 14 | 45 ± 14 |
| EBV | 40 ± 8* | 867 ± 65** | 907 ± 73** |
| GM-CSF + EBV | 31 ± 4 | 1944 ± 273* | 1975 ± 274* |
Neutrophils (107/ml) were preincubated with or without 1 nM GM-CSF for 1 h at 37°C and further incubated with or without EBV. Cell-associated and secreted MIP-1α were assessed by a specific ELISA. Results are expressed as pg/ml and are the mean ± SEM of four separate experiments. Values obtained for neutrophils incubated with GM-CSF or EBV alone were compared with those for unstimulated cells, and values for GM-CSF-treated neutrophils stimulated with EBV were compared with the sum of the values obtained when neutrophils were incubated with GM-CSF or EBV alone. *p <0.05, **p <0.01.