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Table II.

RT-PCR analysis of Ras4–12 CTL lines for TCR Vαβ chain usagea

Ras4–12(V12)- Derived TCR VαRas4–12(L12)- Derived TCR VαRas4–12(V12)- Derived TCR VβRas4–12(L12)- Derived TCR Vβ
Vα1 +++ +++ Vβ1 − − 
Vα2 − − Vβ2 − 
Vα3 − − Vβ3 − − 
Vα4 − Vβ4 − − 
Vα5 − − Vβ5.1 − − 
Vα6 − − Vβ5.2 − − 
Vα7 − − Vβ6 − 
Vα8 − Vβ7 − − 
Vα9 − − Vβ8 − − 
Vα10 − − Vβ9 +++ +++ 
Vα11 − − Vβ10b − − 
Vα12 − − Vβ11 − − 
Vα13 − − Vβ12 − − 
VαA10 − − Vβ13 − − 
VαBWB − − Vβ14 − − 
VαBMA − − Vβ15 − − 
VαBMB − − Vβ16 − − 
Vα34S − − Vβ17 − − 
Vα5T − − Vβ18 − − 
   Vβ19 − − 
Ras4–12(V12)- Derived TCR VαRas4–12(L12)- Derived TCR VαRas4–12(V12)- Derived TCR VβRas4–12(L12)- Derived TCR Vβ
Vα1 +++ +++ Vβ1 − − 
Vα2 − − Vβ2 − 
Vα3 − − Vβ3 − − 
Vα4 − Vβ4 − − 
Vα5 − − Vβ5.1 − − 
Vα6 − − Vβ5.2 − − 
Vα7 − − Vβ6 − 
Vα8 − Vβ7 − − 
Vα9 − − Vβ8 − − 
Vα10 − − Vβ9 +++ +++ 
Vα11 − − Vβ10b − − 
Vα12 − − Vβ11 − − 
Vα13 − − Vβ12 − − 
VαA10 − − Vβ13 − − 
VαBWB − − Vβ14 − − 
VαBMA − − Vβ15 − − 
VαBMB − − Vβ16 − − 
Vα34S − − Vβ17 − − 
Vα5T − − Vβ18 − − 
   Vβ19 − − 
a

CTL lines were subjected to RT-PCR analysis for Vαβ chain usage as described in Materials and Methods. PCR products were electrophoresed on 4% agarose gels and scored positive for appropriately sized bands. Predominant Vαβ chains (+++) are indicated as the most intensely stained bands; minor Vαβ chains (+) stained less intensely. Negative lanes (−) had either no detectable bands or nonspecific bands.

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