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Table I.

Specificity of the TGF-β assaya

Expt.Anti-CD2Medium (pg/ml)Anti-TGF-β (pg/ml)mIgG1
− 229 <5 279 
 593 <5 284 
− 210 <5 360 
 445 <5 288 
− 722 <5 764 
 1365 <5 1359 
− 3807 <5 2121 
 >8000 96 3886 
Expt.Anti-CD2Medium (pg/ml)Anti-TGF-β (pg/ml)mIgG1
− 229 <5 279 
 593 <5 284 
− 210 <5 360 
 445 <5 288 
− 722 <5 764 
 1365 <5 1359 
− 3807 <5 2121 
 >8000 96 3886 
a

PBL (1 × 105 were incubated with or without anti-CD2 for 48 h, and the supernatants were harvested. Anti-TGF-β (10 μg/ml) or an equivalent amount of control mouse IgG1 was added, and the supernatant was examined for total TGF-β activity using mink cells (MLEC) with a luciferase construct.

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