The sequence of the whole fusion protein encoded by the pBM163 clone, deduced from nucleotide sequence (GenBank accession number AF043380), tandemly includes GST (italic), a cleavage site for Factor Xa protease (∗), and the filaggrin unit (2-323). In the filaggrin unit, by comparison to the previously published consensus sequence (24), the NH₂-terminal and COOH-terminal aa are lacking. The positions of the COOH-terminal aa of the eight polypeptides generated by site-specific proteolytic cleavage of the filaggrin unit, approximately localized according to the molecular mass of the peptides estimated by SDS-PAGE (Fig. 3, lanes C), are underlined (bold-type numbers 2–9). Standard one-letter code is used for aa and, in the filaggrin sequence, arginines are bold-typed.