Filaggrin-derived synthetic peptides
| Peptide . | Sequencea . | Positionb (homology with consensusc filaggrind ) . | Positionc (homology with recombinant filaggrind ) . |
|---|---|---|---|
| E12D/E12Dcit | ESSRDGSR/citHPRSHD | 108(57), 113(36), 184(86) | 22(36), 108(57), 113(36), 184(71), 237(50) |
| T12E/T12Ecit | TGSSTGGR/citQGSHHE | 18(93), 53(86) | 18(79), 53(79), 311(36) |
| E12H/E12Hcit | EQSADSSR/citHSGSGH | 31(64), 108(79), 125(57), 204(64), 246(86) | 31(57), 108(79), 125(50), 204(57) |
| Peptide . | Sequencea . | Positionb (homology with consensusc filaggrind ) . | Positionc (homology with recombinant filaggrind ) . |
|---|---|---|---|
| E12D/E12Dcit | ESSRDGSR/citHPRSHD | 108(57), 113(36), 184(86) | 22(36), 108(57), 113(36), 184(71), 237(50) |
| T12E/T12Ecit | TGSSTGGR/citQGSHHE | 18(93), 53(86) | 18(79), 53(79), 311(36) |
| E12H/E12Hcit | EQSADSSR/citHSGSGH | 31(64), 108(79), 125(57), 204(64), 246(86) | 31(57), 108(79), 125(50), 204(57) |
Standard one-letter code is used for aa residues except for citrulline, which is abbreviated as cit. The central pentapeptides chosen as potential epitopes are bold-typed.
The various positions of the 14-aa peptides on the consensus sequence of filaggrin and on the filaggrin unit encoded by the pBM163 clone are indicated by the number of the first aa.
The consensus sequence of filaggrin was determined from all the various published sequences of genomic DNA and cDNA clones (24, 35) and from that of the pBM163 clone (Table I).
At each position, the percentage of homology with the synthesized peptide is shown in parentheses.