ELISA reactivity to the citrulline-substituted synthetic peptides of AFA purified from sera of RA patientsa
| Purified AFA . | E12Dcit . | T12Ecit . | E12Hcit . |
|---|---|---|---|
| AFAp | 460 | 0 | 1566 |
| 1 | 201 | 0 | 1219 |
| 2 | 111 | 0 | 154 |
| 3 | 864 | 0 | 0 |
| 4 | 0 | 0 | 131 |
| 5 | 0 | 0 | 0 |
| 6 | 376 | 0 | 1786 |
| 7 | 150 | 0 | 717 |
| 8 | 903 | 0 | 1073 |
| 9 | 0 | 0 | 0 |
| 10 | 174 | 0 | 1069 |
| 11 | 0 | 0 | 102 |
| 12 | 190 | 0 | 1725 |
| Purified AFA . | E12Dcit . | T12Ecit . | E12Hcit . |
|---|---|---|---|
| AFAp | 460 | 0 | 1566 |
| 1 | 201 | 0 | 1219 |
| 2 | 111 | 0 | 154 |
| 3 | 864 | 0 | 0 |
| 4 | 0 | 0 | 131 |
| 5 | 0 | 0 | 0 |
| 6 | 376 | 0 | 1786 |
| 7 | 150 | 0 | 717 |
| 8 | 903 | 0 | 1073 |
| 9 | 0 | 0 | 0 |
| 10 | 174 | 0 | 1069 |
| 11 | 0 | 0 | 102 |
| 12 | 190 | 0 | 1725 |
The values shown are the differences between OD (× 103) on citrulline-substituted and unsubstituted peptides. Only differences above 100 were considered positive. AFAp, pool of AFA purified from 45 different sera; 1–12, AFA purified from 12 different sera.