Skip to Main Content
Table I.

TCRBV region usage, determined by quantitative RT-PCR, and the number of T cell clonotypes, determined by TCR clonotype mapping, in s.c. melanoma lesions from patients 1 and 2a

TCRBVPatient 1Patient 2
Lesion 1Lesion 2Lesion 3PBLsLesion 1Lesion 2Lesion 3PBLs
Exp. %ClonesExp. %ClonesExp. %ClonesExp. %Exp. %ClonesExp. %ClonesExp. %ClonesExp. %
3.7 5.7 4.6 >4 4.1 (0.2) 3.5 (1.0) 8.4 (1.3) 8.1 (0.0) 9.2 (1.1) 
4.1 7.9 >4 3.0 3.3 (0.5) 2.7 (0.0) 3 5.4 (1.6) 1 5.0 (0.3) 3 5.9 (1.5) 
6.4 7.4 5.2 >4 7.0 (0.0) 6.6 (1.4) >4 10.6 (0.4) 3.4 (0.3) 3.1 (1.6) 
5.4 >4 9.8 2.3 3.3 (0.7) 6.4 (0.2) 4.0 (0.0) 7.9 (1.1) 5.7 (0.4) 
4.0 9.3 4.5 3.8 (0.1) 9.0 (1.6) >4 7.6 (2.8) 3.9 (0.0) 4.6 (2.2) 
2.8 10.1 6.0 4.2 (1.7) 4.7 (0.1) 4.0 (1.0) 6.9 (0.1) 3.9 (0.2) 
<1 <1 <1 1.5 (0.5) <1 (0.0) <1 (0.1) <1 (0.1) 4.6 (0.6) 
6.7 >4 9.5 2.9 >4 6.2 (0.7) 8.1 (0.5) 9.1 (0.7) 4 9.0 (2.1) 4 3.3 (0.4) 
3.5 2.9 3.1 4.0 (0.3) 2.5 (0.0) 6.3 (2.5) 1.8 (0.1) 4.4 (0.5) 
10 5.6 <1 3.5 4.0 (0.6) 7.3 (0.2) <1 (0.1) 1.4 (0.1) 3.7 (0.4) 
11 <1 <1 3.0 2.9 (0.6) 6.7 (1.4) <1 (0.1) <1 (0.1) 2.6 (0.8) 
12 3.8 <1 3.5 4.8 (0.8) 4.5 (1.6) >4 <1 (0.2) 7.2 (1.7) 3.9 (1.6) 
13 4.2 >4 3.8 2.7 >4 4.3 (1.9) 4.2 (1.2) >4 7.3 (0.2) <4 5.1 (0.7) 3 7.5 (0.7) 
14 6.3 >4 1.1 3.8 3.7 (0.3) 3.9 (1.1) >4 2.5 (0.0) 2 3.6 (1.0) 2 3.4 (0.8) 
15 1.5 <1 1.5 >4 2.5 (0.2) <1 (0.1) 1.5 (0.1) <1 (0.6) 1.5 (0.8) 
16 3.8 4.3 4.6 >4 3.2 (1.5) 2.6 (0.0) 1.0 (0.3) 1.6 (0.4) 2.7 (0.5) 
17 2.9 >4 <1 2.8 4.0 (0.2) 2.1 (0.3) 2.9 (0.1) 1.1 (0.2) 5.4 (0.8) 
18 2.8 2.3 2.9 4.5 (0.5) 1.9 (0.2) 5.3 (1.3) 1.9 (1.2) 3.4 (0.8) 
19 4.1 <1 4.4 4.3 (0.2) 3.4 (0.3) 6.5 (1.2) <1 (0.1) 2.2 (0.3) 
20 7.1 8.1 8.6 6.5 (0.4) <1 (0.0) 8.6 (0.7) 7.2 (1.0) 2.0 (0.1) 
21 4.6 4.1 >4 6.3 6.4 (1.3) 5.4 (2.1) 5.3 (0.3) 6.4 (0.6) 7.3 (1.0) 
22 8.2 >4 7.6 8.6 5.0 (2.4) 6.3 (0.1) 2.1 (0.2) 6.9 (0.1) 4.3 (1.8) 
23 2.7 >4 <1 4.2 1.9 (0.3) 2.8 (0.0) <1 (0.0) 2.7 (0.1) 5.1 (0.2) 
24 5.1 >4 3.3 7.2 >4 4.4 (1.2) 3.7 (1.1) <1 (0.0) 7.3 (0.3) 0.9 (0.0) 
TCRBVPatient 1Patient 2
Lesion 1Lesion 2Lesion 3PBLsLesion 1Lesion 2Lesion 3PBLs
Exp. %ClonesExp. %ClonesExp. %ClonesExp. %Exp. %ClonesExp. %ClonesExp. %ClonesExp. %
3.7 5.7 4.6 >4 4.1 (0.2) 3.5 (1.0) 8.4 (1.3) 8.1 (0.0) 9.2 (1.1) 
4.1 7.9 >4 3.0 3.3 (0.5) 2.7 (0.0) 3 5.4 (1.6) 1 5.0 (0.3) 3 5.9 (1.5) 
6.4 7.4 5.2 >4 7.0 (0.0) 6.6 (1.4) >4 10.6 (0.4) 3.4 (0.3) 3.1 (1.6) 
5.4 >4 9.8 2.3 3.3 (0.7) 6.4 (0.2) 4.0 (0.0) 7.9 (1.1) 5.7 (0.4) 
4.0 9.3 4.5 3.8 (0.1) 9.0 (1.6) >4 7.6 (2.8) 3.9 (0.0) 4.6 (2.2) 
2.8 10.1 6.0 4.2 (1.7) 4.7 (0.1) 4.0 (1.0) 6.9 (0.1) 3.9 (0.2) 
<1 <1 <1 1.5 (0.5) <1 (0.0) <1 (0.1) <1 (0.1) 4.6 (0.6) 
6.7 >4 9.5 2.9 >4 6.2 (0.7) 8.1 (0.5) 9.1 (0.7) 4 9.0 (2.1) 4 3.3 (0.4) 
3.5 2.9 3.1 4.0 (0.3) 2.5 (0.0) 6.3 (2.5) 1.8 (0.1) 4.4 (0.5) 
10 5.6 <1 3.5 4.0 (0.6) 7.3 (0.2) <1 (0.1) 1.4 (0.1) 3.7 (0.4) 
11 <1 <1 3.0 2.9 (0.6) 6.7 (1.4) <1 (0.1) <1 (0.1) 2.6 (0.8) 
12 3.8 <1 3.5 4.8 (0.8) 4.5 (1.6) >4 <1 (0.2) 7.2 (1.7) 3.9 (1.6) 
13 4.2 >4 3.8 2.7 >4 4.3 (1.9) 4.2 (1.2) >4 7.3 (0.2) <4 5.1 (0.7) 3 7.5 (0.7) 
14 6.3 >4 1.1 3.8 3.7 (0.3) 3.9 (1.1) >4 2.5 (0.0) 2 3.6 (1.0) 2 3.4 (0.8) 
15 1.5 <1 1.5 >4 2.5 (0.2) <1 (0.1) 1.5 (0.1) <1 (0.6) 1.5 (0.8) 
16 3.8 4.3 4.6 >4 3.2 (1.5) 2.6 (0.0) 1.0 (0.3) 1.6 (0.4) 2.7 (0.5) 
17 2.9 >4 <1 2.8 4.0 (0.2) 2.1 (0.3) 2.9 (0.1) 1.1 (0.2) 5.4 (0.8) 
18 2.8 2.3 2.9 4.5 (0.5) 1.9 (0.2) 5.3 (1.3) 1.9 (1.2) 3.4 (0.8) 
19 4.1 <1 4.4 4.3 (0.2) 3.4 (0.3) 6.5 (1.2) <1 (0.1) 2.2 (0.3) 
20 7.1 8.1 8.6 6.5 (0.4) <1 (0.0) 8.6 (0.7) 7.2 (1.0) 2.0 (0.1) 
21 4.6 4.1 >4 6.3 6.4 (1.3) 5.4 (2.1) 5.3 (0.3) 6.4 (0.6) 7.3 (1.0) 
22 8.2 >4 7.6 8.6 5.0 (2.4) 6.3 (0.1) 2.1 (0.2) 6.9 (0.1) 4.3 (1.8) 
23 2.7 >4 <1 4.2 1.9 (0.3) 2.8 (0.0) <1 (0.0) 2.7 (0.1) 5.1 (0.2) 
24 5.1 >4 3.3 7.2 >4 4.4 (1.2) 3.7 (1.1) <1 (0.0) 7.3 (0.3) 0.9 (0.0) 
a

Each BV region signal (Exp. %) is expressed as the mean percentage (SD) of the total TCRBV signal. Underlined values represent BV regions expressed at a frequency of 1.8 above the frequency in PBL. Values of <1% are indicated as <1. When more than four clonotypic transcripts were detected by clonotype mapping, this is designated as >4. No clonal transcripts were detected in the PBLs. BV-specific Abs were used to stain the regions shown in bold.

Close Modal

or Create an Account

Close Modal
Close Modal