Sections of oxazolone-inflamed ears were prepared and stained for two-color immunohistochemistry as described in Materials and Methods. Control staining with normal rat IgG and without primary Abs demonstrated the specificity of the staining patterns and indicated that no cross-reactivity between color reactions developed.

Percentages represent the fraction of total CD8⁺ T cells that each subset comprises and were counted from >120 ×40 sections using one to two Tg mice in each of three independent experiments.