Ability of PECAM-1-specific Abs to induce calcium mobilization or inhibit TCR-mediated calcium mobilization in Jurkat T lymphocytes
| Anti-PECAM-1 mAb . | Ig Domain Specificity . | Induction of Ca2+ Flux . | Inhibition of TCR-Mediated Ca2+ Flux . |
|---|---|---|---|
| PECAM-1.1 | 5 | No | Noa |
| PECAM-1.2 | 6 | No | Yes |
| PECAM-1.3 | 1 | No | Yes |
| 4G6 | 6 | No | Yes |
| Anti-PECAM-1 mAb . | Ig Domain Specificity . | Induction of Ca2+ Flux . | Inhibition of TCR-Mediated Ca2+ Flux . |
|---|---|---|---|
| PECAM-1.1 | 5 | No | Noa |
| PECAM-1.2 | 6 | No | Yes |
| PECAM-1.3 | 1 | No | Yes |
| 4G6 | 6 | No | Yes |
Levels of PECAM-1.1 binding to cell surface PECAM-1 and of GAM binding to surface-bound PECAM-1.1, as assessed by flow cytometry, did not differ from those observed with PECAM-1.2 and PECAM-1.3 Abs (data not shown).