Phenotypic comparison of SW480 to SW620 colon carcinoma cells before and after IFN-γ pretreatmenta
| Tumor Cell Line . | IFN-γ Pretreatment . | mAb Directed Against % Positive Cells (MFI) . | . | . | . | |||
|---|---|---|---|---|---|---|---|---|
| . | . | HLA-A2 . | ICAM-1 . | LFA-3 . | Fas . | |||
| SW480 | Untreated | 72 ± 8 | 39 ± 6 | 96 ± 0.4 | 13 ± 4 | |||
| (68 ± 7) | (42 ± 6) | (114 ± 9) | (30 ± 2) | |||||
| Pretreated | 97 ± 1 | 98 ± 1 | 96 ± 0.4 | 65 ± 4 | ||||
| (544 ± 32) | (289 ± 35) | (90 ± 9) | (41 ± 3) | |||||
| SW620 | Untreated | 96 ± 1 | 53 ± 8 | 97 ± 0.4 | 2 ± 0.4 | |||
| (100 ± 10) | (35 ± 5) | (92 ± 5) | (18 ± 2) | |||||
| Pretreated | 97 ± 0.4 | 95 ± 1 | 96 ± 1 | 15 ± 4 | ||||
| (499 ± 23) | (214 ± 26) | (70 ± 3) | (20 ± 1) | |||||
| Tumor Cell Line . | IFN-γ Pretreatment . | mAb Directed Against % Positive Cells (MFI) . | . | . | . | |||
|---|---|---|---|---|---|---|---|---|
| . | . | HLA-A2 . | ICAM-1 . | LFA-3 . | Fas . | |||
| SW480 | Untreated | 72 ± 8 | 39 ± 6 | 96 ± 0.4 | 13 ± 4 | |||
| (68 ± 7) | (42 ± 6) | (114 ± 9) | (30 ± 2) | |||||
| Pretreated | 97 ± 1 | 98 ± 1 | 96 ± 0.4 | 65 ± 4 | ||||
| (544 ± 32) | (289 ± 35) | (90 ± 9) | (41 ± 3) | |||||
| SW620 | Untreated | 96 ± 1 | 53 ± 8 | 97 ± 0.4 | 2 ± 0.4 | |||
| (100 ± 10) | (35 ± 5) | (92 ± 5) | (18 ± 2) | |||||
| Pretreated | 97 ± 0.4 | 95 ± 1 | 96 ± 1 | 15 ± 4 | ||||
| (499 ± 23) | (214 ± 26) | (70 ± 3) | (20 ± 1) | |||||
Tumor cells, either untreated or pretreated with IFN-γ, were evaluated by flow cytometry for cell-surface expression of the indicated molecule. Data were expressed as the mean ± SEM of the percentage positive cells and MFI (in parentheses) of 8 (for HLA-A2, ICAM-1), 4 (for LFA-3), and 12 (for Fas) separate experiments. Staining with the appropriate isotype control Ab, which ranged from 2 to 10%, was subtracted from the values shown above. Data in bold type indicate statistically significant responses, based on comparison with the corresponding untreated preparations. For SW480 cells: HLA-A2: percentage positive cells, p < 0.02; MFI, p < 0.0001; ICAM-1: percentage positive cells, p < 0.0001; MFI, p < 0.0001; Fas: percentage positive cells, p < 0.0001. For SW620 cells: HLA-A2: MFI, p < 0.0001; ICAM-1: percentage positive cells, p < 0.001; MFI, p < 0.001; Fas: percentage positive cells, p < 0.005.