Cellular and molecular components essential for the formation of FDC/GC complexa
| Donor BM Cells . | Recipientb . | Chimeric Mice Cells Expressing LT/TNF . | FDC . | GC . | IgG . | Reference . |
|---|---|---|---|---|---|---|
| wt | wt | T, B, NK | + | + | + | 21 |
| wt | LTα−/− | T, B, NK | + | + | + | 21 |
| LTα−/− | wt | None | − | − | − | 21 |
| LTα−/−+ RAG−/− | LTα−/− | NK | − | − | − | 22, 23 |
| LTα−/−+ BCR−/− | LTα−/− | NK, T | − | − | − | 22, 23 |
| LTα−/−+ TCR−/− | LTα−/− | NK, B | + | + | + | 22, 23 |
| LTα−/−+ TNF−/− | LTα−/− | NK, T, B | + | + | + | Fig. 3 |
| wt | TNF−/− | T, B, NK | + | + | + | Fig. 1 |
| TNF−/−+ RAG | TNF−/− | NK | − | − | ND | Fig. 2 |
| TNF−/−+ BCR−/− | TNF−/− | NK, T | + | + | + | Fig. 2 |
| TNF−/−+ TCR−/− | TNF−/− | NK, B | + | + | + | Fig. 2 |
| TNF−/−+ LTα−/− | TNF−/− | NK, T, B | + | + | + | Fig. 3 |
| TNF−/−+ wt Thyc | RAG-1−/− | T | + | + | ND | Fig. 4 |
| TNF−/−+ LTα−/− Thyc | RAG-1−/− | LT−/− T | + | + | ND | Fig. 4 |
| TNF−/− | RAG-1−/− | None | − | − | +/− | Fig. 4 |
| Donor BM Cells . | Recipientb . | Chimeric Mice Cells Expressing LT/TNF . | FDC . | GC . | IgG . | Reference . |
|---|---|---|---|---|---|---|
| wt | wt | T, B, NK | + | + | + | 21 |
| wt | LTα−/− | T, B, NK | + | + | + | 21 |
| LTα−/− | wt | None | − | − | − | 21 |
| LTα−/−+ RAG−/− | LTα−/− | NK | − | − | − | 22, 23 |
| LTα−/−+ BCR−/− | LTα−/− | NK, T | − | − | − | 22, 23 |
| LTα−/−+ TCR−/− | LTα−/− | NK, B | + | + | + | 22, 23 |
| LTα−/−+ TNF−/− | LTα−/− | NK, T, B | + | + | + | Fig. 3 |
| wt | TNF−/− | T, B, NK | + | + | + | Fig. 1 |
| TNF−/−+ RAG | TNF−/− | NK | − | − | ND | Fig. 2 |
| TNF−/−+ BCR−/− | TNF−/− | NK, T | + | + | + | Fig. 2 |
| TNF−/−+ TCR−/− | TNF−/− | NK, B | + | + | + | Fig. 2 |
| TNF−/−+ LTα−/− | TNF−/− | NK, T, B | + | + | + | Fig. 3 |
| TNF−/−+ wt Thyc | RAG-1−/− | T | + | + | ND | Fig. 4 |
| TNF−/−+ LTα−/− Thyc | RAG-1−/− | LT−/− T | + | + | ND | Fig. 4 |
| TNF−/− | RAG-1−/− | None | − | − | +/− | Fig. 4 |
The protocols for BM or thymocytes (Thy) transfer were described in Materials and Methods of relevant references. +, Detectable; +/−, weak positive; −, undetectable.
The recipient except RAG-1−/− mice were lethally irradiated (1050 rad) and received BM cells (see references for detail). RAG-1−/− mice do not receive radiation.
Donor cells are the mixture of TNF-deficient splenocytes plus wt or LT-deficient thymocytes (Fig. 4).