Frequency of VκL chain gene usage in PC-specific splenic B cells from T15i KI mice
| Vκ Genes . | Single Anti-PC Ab-Secreting B Cell Clonesa . | . | |
|---|---|---|---|
| . | T15-Id− . | T15-Id+ . | |
| Vκ22 | 1 (3) | 44 (94) | |
| Vκ8 | 25 (64) | 0 (0) | |
| Vκ19 | 13 (33) | 3 (6) | |
| Vκ Genes . | Single Anti-PC Ab-Secreting B Cell Clonesa . | . | |
|---|---|---|---|
| . | T15-Id− . | T15-Id+ . | |
| Vκ22 | 1 (3) | 44 (94) | |
| Vκ8 | 25 (64) | 0 (0) | |
| Vκ19 | 13 (33) | 3 (6) | |
RNA from 39 clones derived from T15-Id− PC-dex+-sorted cells and 47 clones derived from T15-Id+ PC-dex+-sorted cells was amplified by RT-PCR and sequenced as described in Materials and Methods. The percentage of total clones is listed in parentheses.