Receptor distribution in circulating monocytes stimulated by C. burnetiia
| . | Beads Out of Deformations . | Beads in Deformations . |
|---|---|---|
| CD18 | 76 | 8 (10%)∗ |
| CD11b | 77 | 10 (11%)∗ |
| αvβ3 integrin | 55 | 22 (29%) |
| IAP | 57 | 24 (30%) |
| . | Beads Out of Deformations . | Beads in Deformations . |
|---|---|---|
| CD18 | 76 | 8 (10%)∗ |
| CD11b | 77 | 10 (11%)∗ |
| αvβ3 integrin | 55 | 22 (29%) |
| IAP | 57 | 24 (30%) |
Circulating monocytes were incubated with C. burnetii (bacterium-to-cell ratio of 100:1) for 15 min at 37°C and the distribution of monocyte receptors was assessed using 2.8-μm beads and F-actin labeling. The number of beads bound to CD18, CD11b, αvβ3 integrin, or IAP was determined in and out of the protrusions. The percentage of beads in deformation areas is presented in parentheses.
∗, p < 0.05 represents the comparison between the distribution of CD18 and CD11b, and that of αvβ3 integrin and IAP.