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Table I.

Primers and probes used for real-time quantitative PCRa

Forward PrimerProbeReverse Primer
TLR1 TTCACAGTGTCTGGTACACGCAT TGCCCATCCAAAATTAGCCCGTTCC ACCGTGTCTGTTAAGAGATTATTGGA 
TLR2 GAAGAATCCTCCAATCAGGCTTC CTGTCTTGTGACCGCAATGGTATCTGCA GCCCTGAGGGAATGGAGTTT 
TLR3 GCCGCCAACTTCACAAGGTATA AGCCAGAATTGTGCCAGAAACTTCCCA AGCTCATTGTGCTGGAGGTTC 
TLR4 AATCTAGAGCACTTGGACCTTTCC TGCGGGTTCTACATCAAATGCCCCTAC GGGTTCAGGGACAGGTCTAAAGA 
TLR5 CCATAGATTTTTCCTCCAACCAAATA CTCGAGCCCCTACAAGGGAAAACGCT TCATACATTTTCCCCAGTCCACT 
TLR6 CATCCTATTGTGAGTTTCAGGCAT TCAATGATTTCAAGGCCCTGCCCA GCTTCATAGCACTCAATCCCAAG 
TLR7 GGAGGTATTCCCACGAACACC CGAACCTCACCCTCACCATTAACCACAT TGACCCCAGTGGAATAGGTACAC 
TLR8 AAACTTGACCCAACTTCGATACCTAA CCCTCAGGAAGATTAATGCTGCCTGGTT GATCCAGCACCTTCAGATGAGG 
TLR9 GGACCTCTGGTACTGCTTCCA ACGATGCCTTCGTGGTCTTCGACAAA AAGCTCGTTGTACACCCAGTCT 
TLR10 AAGAAAGGTTCCCGCAGACTT ACCCCAGCCACAACGACACTGGAT TGTTATGGCATAGAATCAAAACTCTCA 
Forward PrimerProbeReverse Primer
TLR1 TTCACAGTGTCTGGTACACGCAT TGCCCATCCAAAATTAGCCCGTTCC ACCGTGTCTGTTAAGAGATTATTGGA 
TLR2 GAAGAATCCTCCAATCAGGCTTC CTGTCTTGTGACCGCAATGGTATCTGCA GCCCTGAGGGAATGGAGTTT 
TLR3 GCCGCCAACTTCACAAGGTATA AGCCAGAATTGTGCCAGAAACTTCCCA AGCTCATTGTGCTGGAGGTTC 
TLR4 AATCTAGAGCACTTGGACCTTTCC TGCGGGTTCTACATCAAATGCCCCTAC GGGTTCAGGGACAGGTCTAAAGA 
TLR5 CCATAGATTTTTCCTCCAACCAAATA CTCGAGCCCCTACAAGGGAAAACGCT TCATACATTTTCCCCAGTCCACT 
TLR6 CATCCTATTGTGAGTTTCAGGCAT TCAATGATTTCAAGGCCCTGCCCA GCTTCATAGCACTCAATCCCAAG 
TLR7 GGAGGTATTCCCACGAACACC CGAACCTCACCCTCACCATTAACCACAT TGACCCCAGTGGAATAGGTACAC 
TLR8 AAACTTGACCCAACTTCGATACCTAA CCCTCAGGAAGATTAATGCTGCCTGGTT GATCCAGCACCTTCAGATGAGG 
TLR9 GGACCTCTGGTACTGCTTCCA ACGATGCCTTCGTGGTCTTCGACAAA AAGCTCGTTGTACACCCAGTCT 
TLR10 AAGAAAGGTTCCCGCAGACTT ACCCCAGCCACAACGACACTGGAT TGTTATGGCATAGAATCAAAACTCTCA 
a

All sequences are presented in the 5′–3′ direction.

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