American Association of Immunologists

Table I.

Subclass and specificity of anti-ricin Abs^a

	Isotype	A Chain	Holotoxin	B Chain	BSA	Western Blot Reactivity
Fusion 1: immunize with A chain, select for A chain binding
RAC mAb 1	IgG2b	0.33	1.00	0.11	0.02	+
RAC mAb 3	IgG1	2.26	2.29	0.07	0.03	+
RAC mAb 4	IgG1	0.36	0.24	0.03	0.01	+
RAC mAb 5	IgG1	0.22	0.95	0.02	0.01	+
RAC mAb 6	IgG2a	0.15	0.18	0.02	0.03	+
RAC mAb 8	IgG1	2.53	2.43	0.09	0.02	+
RAC mAb 9	IgG2b	2.30	2.40	0.58	0.08	+
RAC mAb 10	IgG1	0.83	1.51	0.02	0.03	+
RAC mAb 11	IgG1	0.79	0.54	0.03	0.03	+
RAC mAb 12	IgG2b	1.10	0.99	0.45	0.04	+/−
RAC mAb 14	IgG1	0.42	1.04	0.02	0.02	+
RAC mAb 16	IgG1	2.30	2.27	0.06	0.03	+
RAC mAb 17	IgG1	2.38	2.33	0.13	0.05	+
RAC mAb 18	IgG2a	2.29	2.56	0.06	0.03	+
RAC mAb 19	IgG1	0.61	1.41	0.03	0.02	+/−
RAC mAb 20	IgG2b	0.59	1.35	0.05	0.01	+
RAC mAb 21	IgG1	0.18	0.43	0.04	0.03	−
RAC mAb 22	IgG1	2.54	2.45	0.11	0.05	+
RAC mAb 23	IgG1	2.19	2.38	0.04	0.03	+
Fusion 2: immunize with A chain, then holotoxin, select for survival in ricin
RAC mAb 24	IgG2a	0.74	1.51	0.03	0.01	+/−
Holo mAb 1	IgG1	0.18	0.57	0.02	0.01	−
HAB mAb 1	IgG1	0.85	1.13	0.93	0.01	−
Fusion 3: immunize with B chain, select for B chain binding
RBC mAb 1	IgG1	0.14	0.99	1.17	0.06	−
RBC mAb 2	IgG1	0.01	0.44	0.35	0.03	−
RBC mAb 3	IgG1	0.01	0.07	0.25	0.03	−
RBC mAb 4	IgG2a	0.48	1.95	2.14	0.03	+
RBC mAb 5	IgG1	0.19	1.50	1.64	0.03	+
RBC mAb 6	IgG1	0.02	0.48	0.93	0.04	−
RBC mAb 7	IgG2b	0.10	0.99	1.37	0.05	+
RBC mAb 8	IgG2b	0.01	1.73	1.82	0.02	−
RBC mAb 9	IgG2a	0.00	0.07	0.14	0.02	−
RBC mAb 10	IgG2b	0.58	1.96	2.02	0.03	+
RBC mAb 11	IgG2b	0.50	2.09	2.03	0.04	+
RBC mAb 12	IgG2a	0.00	0.22	0.37	0.08	−
RBC mAb 13	IgG1	0.01	0.16	0.16	0.04	−
RBC mAb 14	IgG2b	0.49	1.96	2.15	0.03	+
RBC mAb 15	IgG2a	0.18	1.76	1.60	0.08	+
RBC mAb 16	IgG2a	0.02	0.88	1.35	0.05	+
RBC mAb 17	IgG1	0.40	1.90	1.79	0.01	+
RBC mAb 18	IgG1	0.17	1.21	1.35	0.01	−
Medium		0.02	0.04	0.01	0.01
Irrel mAb		0.04	0.07	0.02	0.02

	Isotype	A Chain	Holotoxin	B Chain	BSA	Western Blot Reactivity
Fusion 1: immunize with A chain, select for A chain binding
RAC mAb 1	IgG2b	0.33	1.00	0.11	0.02	+
RAC mAb 3	IgG1	2.26	2.29	0.07	0.03	+
RAC mAb 4	IgG1	0.36	0.24	0.03	0.01	+
RAC mAb 5	IgG1	0.22	0.95	0.02	0.01	+
RAC mAb 6	IgG2a	0.15	0.18	0.02	0.03	+
RAC mAb 8	IgG1	2.53	2.43	0.09	0.02	+
RAC mAb 9	IgG2b	2.30	2.40	0.58	0.08	+
RAC mAb 10	IgG1	0.83	1.51	0.02	0.03	+
RAC mAb 11	IgG1	0.79	0.54	0.03	0.03	+
RAC mAb 12	IgG2b	1.10	0.99	0.45	0.04	+/−
RAC mAb 14	IgG1	0.42	1.04	0.02	0.02	+
RAC mAb 16	IgG1	2.30	2.27	0.06	0.03	+
RAC mAb 17	IgG1	2.38	2.33	0.13	0.05	+
RAC mAb 18	IgG2a	2.29	2.56	0.06	0.03	+
RAC mAb 19	IgG1	0.61	1.41	0.03	0.02	+/−
RAC mAb 20	IgG2b	0.59	1.35	0.05	0.01	+
RAC mAb 21	IgG1	0.18	0.43	0.04	0.03	−
RAC mAb 22	IgG1	2.54	2.45	0.11	0.05	+
RAC mAb 23	IgG1	2.19	2.38	0.04	0.03	+
Fusion 2: immunize with A chain, then holotoxin, select for survival in ricin
RAC mAb 24	IgG2a	0.74	1.51	0.03	0.01	+/−
Holo mAb 1	IgG1	0.18	0.57	0.02	0.01	−
HAB mAb 1	IgG1	0.85	1.13	0.93	0.01	−
Fusion 3: immunize with B chain, select for B chain binding
RBC mAb 1	IgG1	0.14	0.99	1.17	0.06	−
RBC mAb 2	IgG1	0.01	0.44	0.35	0.03	−
RBC mAb 3	IgG1	0.01	0.07	0.25	0.03	−
RBC mAb 4	IgG2a	0.48	1.95	2.14	0.03	+
RBC mAb 5	IgG1	0.19	1.50	1.64	0.03	+
RBC mAb 6	IgG1	0.02	0.48	0.93	0.04	−
RBC mAb 7	IgG2b	0.10	0.99	1.37	0.05	+
RBC mAb 8	IgG2b	0.01	1.73	1.82	0.02	−
RBC mAb 9	IgG2a	0.00	0.07	0.14	0.02	−
RBC mAb 10	IgG2b	0.58	1.96	2.02	0.03	+
RBC mAb 11	IgG2b	0.50	2.09	2.03	0.04	+
RBC mAb 12	IgG2a	0.00	0.22	0.37	0.08	−
RBC mAb 13	IgG1	0.01	0.16	0.16	0.04	−
RBC mAb 14	IgG2b	0.49	1.96	2.15	0.03	+
RBC mAb 15	IgG2a	0.18	1.76	1.60	0.08	+
RBC mAb 16	IgG2a	0.02	0.88	1.35	0.05	+
RBC mAb 17	IgG1	0.40	1.90	1.79	0.01	+
RBC mAb 18	IgG1	0.17	1.21	1.35	0.01	−
Medium		0.02	0.04	0.01	0.01
Irrel mAb		0.04	0.07	0.02	0.02

The specifity of each Ab was tested in two to nine different experiments, subclass in one to three. Data shown are representative of those experiments.

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