American Association of Immunologists

Table I.

Proliferative response of CD8⁺ thymocytes and lymph node cells from H-2^bxd (InsHA × Clone 4)F₁ mice^a

Responder CD8⁺ T Cells	[³H]Thymidine in Response to APC
Responder CD8⁺ T Cells		H-2^d	H-2^d + K^dHA
Experiment 1
H-2^bxd Clone 4 thymocytes	95	83,561
H-2^bxd (InsHA× Clone 4)F₁ thymocytes	157	136
H-2^bxd Clone 4 lymph node cells	109	153,761
H-2^bxd (InsHA× Clone 4)F₁ lymph node cells	440	293
Experiment 2
H-2^d Clone 4 thymocytes	410	6,739
H-2^bxd Clone 4 thymocytes	556	4,956
H-2^d Clone 4 lymph node cells	188	29,301
H-2^bxd Clone 4 lymph node cells	480	24,884

Responder CD8⁺ T Cells	[³H]Thymidine in Response to APC
Responder CD8⁺ T Cells		H-2^d	H-2^d + K^dHA
Experiment 1
H-2^bxd Clone 4 thymocytes	95	83,561
H-2^bxd (InsHA× Clone 4)F₁ thymocytes	157	136
H-2^bxd Clone 4 lymph node cells	109	153,761
H-2^bxd (InsHA× Clone 4)F₁ lymph node cells	440	293
Experiment 2
H-2^d Clone 4 thymocytes	410	6,739
H-2^bxd Clone 4 thymocytes	556	4,956
H-2^d Clone 4 lymph node cells	188	29,301
H-2^bxd Clone 4 lymph node cells	480	24,884

A total of 5 × 10⁴ responder cells were incubated for 36 h with 5 × 10⁵ irradiated APC pulsed with or without K^dHA peptide, as described under Materials and Methods.

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