Estimate of number of epitope-specific CD8 T cell clonotypes per infected spleen
| Mouse . | Vβ . | Vβ13, Vβ5, or Vβ8 Epitope-Specific Cells/Spleen (no.) . | α(P)a . | Frequency of Vβ13, Vβ5, or Vβ8 clonotypes (per 10,000)b . | Total No. of Vβ13, Vβ5, Vβ8 Clonotypes/Infected Spleen (CI)b . | Total No. of Clonotypes/Spleenc . |
|---|---|---|---|---|---|---|
| JHM S510-1 | Vβ13 | 310,000 | 27.08 (0.60) | 160 | 253 (170–333) | 1,150 |
| Vβ8 | 197,000 | 22.80 (0.043) | 139 | 206 (139–271) | 1,471 | |
| S510-2 | Vβ13 | 438,000 | 35.42 (0.73) | 200 | 334 (226–437) | 1,518 |
| JHM S598-1 | Vβ5 | 160,000 | 24.70 (0.87) | 148 | 217 (141–289) | 775 |
| S598-2 | Vβ5 | 350,000 | 26.27 (0.23) | 156 | 249 (163–332) | 889 |
| S598-3 | Vβ5 | 1,030,000 | 14.59 (0.24) | 95 | 163 (100–224) | 582 |
| LCMV gp33–1 | Vβ8 | 1,280,000 | 29.5 (0.95) | 172 | 315 (223–404) | 1,050 |
| gp33-2 | Vβ8 | 2,510,000 | 32.7 (0.78) | 187 | 368 (264–469) | 1,227 |
| Mouse . | Vβ . | Vβ13, Vβ5, or Vβ8 Epitope-Specific Cells/Spleen (no.) . | α(P)a . | Frequency of Vβ13, Vβ5, or Vβ8 clonotypes (per 10,000)b . | Total No. of Vβ13, Vβ5, Vβ8 Clonotypes/Infected Spleen (CI)b . | Total No. of Clonotypes/Spleenc . |
|---|---|---|---|---|---|---|
| JHM S510-1 | Vβ13 | 310,000 | 27.08 (0.60) | 160 | 253 (170–333) | 1,150 |
| Vβ8 | 197,000 | 22.80 (0.043) | 139 | 206 (139–271) | 1,471 | |
| S510-2 | Vβ13 | 438,000 | 35.42 (0.73) | 200 | 334 (226–437) | 1,518 |
| JHM S598-1 | Vβ5 | 160,000 | 24.70 (0.87) | 148 | 217 (141–289) | 775 |
| S598-2 | Vβ5 | 350,000 | 26.27 (0.23) | 156 | 249 (163–332) | 889 |
| S598-3 | Vβ5 | 1,030,000 | 14.59 (0.24) | 95 | 163 (100–224) | 582 |
| LCMV gp33–1 | Vβ8 | 1,280,000 | 29.5 (0.95) | 172 | 315 (223–404) | 1,050 |
| gp33-2 | Vβ8 | 2,510,000 | 32.7 (0.78) | 187 | 368 (264–469) | 1,227 |
Values of p from a G test for goodness-of-fit to the estimated log-series distribution; p < 0.05 indicates significant departure of the data from the log-series distribution.
Numbers assume fit to the estimated log-series distributions. CI, 95% confidence interval.
Calculated assuming that cells expressing Vβ13 or Vβ8 (S510), Vβ5 (S598), or Vβ8 (gp33) comprise 22 or 14% (7 ), 28% (our unpublished data), or 30% (22 ) of epitope S510-, S-598-, or gp33-specific CD8 T cells, respectively.