Frequencies of TRBV20-1 sequences with unique D region in the 1803Tu samplea
| TRBV20-1 Sequence . | Sequence of Unique D Region . | Frequency . |
|---|---|---|
| TRBV20-1-TIL | AGA GGG ACT TCC GGA CT | N/A |
| TRBV20-1-1 | AGA GAT AAC CCC GGG ACT AG | 1/64 |
| TRBV20-1-2 | TGG GGG ACA GG | 2/64 |
| TRBV20-1-3 | AGA GCC GAC AGG GGG AA | 6/64 |
| TRBV20-1-4 | AGT CGG GGG ACA GG | 1/64 |
| TRBV20-1-5 | AGA GGT TGG GAC GAG | 5/64 |
| TRBV20-1-6 | TGG ACA GCG AG | 2/64 |
| TRBV20-1-7 | AGA CGA CAG AAC GG | 1/64 |
| TRBV20-1-8 | AGA GTT GAT AGG A | 1/64 |
| TRBV20-1-9 | AGG ACC GGG ACA GGG | 2/64 |
| TRBV20-1-10 | AGA GGG CCA GGG GC | 2/64 |
| TRBV20-1-11 | CAG CAA CTA GCG GGG | 1/64 |
| TRBV20-1-12 | GAA GAG CCA AAC AGG AGG | 1/64 |
| TRBV20-1-13 | AGA GGG ACT TCC GGA CT | 1/64 |
| TRBV20-1-14 | AGA GAT CGA CAG | 2/64 |
| TRBV20-1-15 | AGA GGC GTT CAG GGG | 1/64 |
| TRBV20-1-16 | AGA GCC GAC AGG GGG | 1/64 |
| TRBV20-1-17 | AGA GAT GGT CCC GGG | 1/64 |
| TRBV20-1-18 | AGT TTC TGG GAC GT | 1/64 |
| TRBV20-1-19 | AGA GAC TCT GGG | 1/64 |
| TRBV20-1-20 | CAG CAC ACT AGC GGG | 1/64 |
| TRBV20-1-21 | AGA GGG CTC AGG GG | 1/64 |
| TRBV20-1-22 | AGA GGG CCA GGG | 1/64 |
| TRBV20-1-23 | AGA GAT TGG TGA GG | 1/64 |
| TRBV20-1-24 | CCT GGC TGG GGG | 1/64 |
| TRBV20-1-25 | AGA GGG GGA CAG GGG | 1/64 |
| TRBV20-1-26 | AGA GAG GGT TCG TG | 1/64 |
| TRBV20-1-27 | AGA GAT TGG GGC GGT TGC C | 1/64 |
| TRBV20-1-28 | TAC TGA CGA GGG ATT GG | 1/64 |
| TRBV20-1-29 | GGT TGG TTG GG | 1/64 |
| TRBV20-1-30 | GGA GGT TGG GA | 1/64 |
| TRBV20-1-31 | GGG GAG GTT GGG | 1/64 |
| TRBV20-1-32 | GCT ACA GGC CCC CT | 1/64 |
| TRBV20-1-33 | AGG CAG GCA TT | 1/64 |
| TRBV20-1-34 | CGG GAC A | 1/64 |
| TRBV20-1-35 | AGA AGT GAT G | 3/64 |
| TRBV20-1-36 | AGA GAT TGG AGC GGA GCA AGC A | 2/64 |
| TRBV20-1-37 | AGA GAT TGG TCG AGA CAA CAG | 1/64 |
| TRBV20-1-38 | GGG ATG GAC ACA T | 1/64 |
| TRBV20-1-39 | CCA GGG GGA AT | 1/64 |
| TRBV20-1-40 | AGA CGG ACA GGG GAG CC | 1/64 |
| TRBV20-1-41 | CCT AGC GGG GGG GC | 3/64 |
| TRBV20-1-42 | AGA GAC TCA GGG TTG AG | 2/64 |
| TRBV20-1-43 | AGA GAC GTG TGG GGG GG | 1/64 |
| TRBV20-1-44 | CCG GGA CAG GG | 1/64 |
| TRBV20-1 Sequence . | Sequence of Unique D Region . | Frequency . |
|---|---|---|
| TRBV20-1-TIL | AGA GGG ACT TCC GGA CT | N/A |
| TRBV20-1-1 | AGA GAT AAC CCC GGG ACT AG | 1/64 |
| TRBV20-1-2 | TGG GGG ACA GG | 2/64 |
| TRBV20-1-3 | AGA GCC GAC AGG GGG AA | 6/64 |
| TRBV20-1-4 | AGT CGG GGG ACA GG | 1/64 |
| TRBV20-1-5 | AGA GGT TGG GAC GAG | 5/64 |
| TRBV20-1-6 | TGG ACA GCG AG | 2/64 |
| TRBV20-1-7 | AGA CGA CAG AAC GG | 1/64 |
| TRBV20-1-8 | AGA GTT GAT AGG A | 1/64 |
| TRBV20-1-9 | AGG ACC GGG ACA GGG | 2/64 |
| TRBV20-1-10 | AGA GGG CCA GGG GC | 2/64 |
| TRBV20-1-11 | CAG CAA CTA GCG GGG | 1/64 |
| TRBV20-1-12 | GAA GAG CCA AAC AGG AGG | 1/64 |
| TRBV20-1-13 | AGA GGG ACT TCC GGA CT | 1/64 |
| TRBV20-1-14 | AGA GAT CGA CAG | 2/64 |
| TRBV20-1-15 | AGA GGC GTT CAG GGG | 1/64 |
| TRBV20-1-16 | AGA GCC GAC AGG GGG | 1/64 |
| TRBV20-1-17 | AGA GAT GGT CCC GGG | 1/64 |
| TRBV20-1-18 | AGT TTC TGG GAC GT | 1/64 |
| TRBV20-1-19 | AGA GAC TCT GGG | 1/64 |
| TRBV20-1-20 | CAG CAC ACT AGC GGG | 1/64 |
| TRBV20-1-21 | AGA GGG CTC AGG GG | 1/64 |
| TRBV20-1-22 | AGA GGG CCA GGG | 1/64 |
| TRBV20-1-23 | AGA GAT TGG TGA GG | 1/64 |
| TRBV20-1-24 | CCT GGC TGG GGG | 1/64 |
| TRBV20-1-25 | AGA GGG GGA CAG GGG | 1/64 |
| TRBV20-1-26 | AGA GAG GGT TCG TG | 1/64 |
| TRBV20-1-27 | AGA GAT TGG GGC GGT TGC C | 1/64 |
| TRBV20-1-28 | TAC TGA CGA GGG ATT GG | 1/64 |
| TRBV20-1-29 | GGT TGG TTG GG | 1/64 |
| TRBV20-1-30 | GGA GGT TGG GA | 1/64 |
| TRBV20-1-31 | GGG GAG GTT GGG | 1/64 |
| TRBV20-1-32 | GCT ACA GGC CCC CT | 1/64 |
| TRBV20-1-33 | AGG CAG GCA TT | 1/64 |
| TRBV20-1-34 | CGG GAC A | 1/64 |
| TRBV20-1-35 | AGA AGT GAT G | 3/64 |
| TRBV20-1-36 | AGA GAT TGG AGC GGA GCA AGC A | 2/64 |
| TRBV20-1-37 | AGA GAT TGG TCG AGA CAA CAG | 1/64 |
| TRBV20-1-38 | GGG ATG GAC ACA T | 1/64 |
| TRBV20-1-39 | CCA GGG GGA AT | 1/64 |
| TRBV20-1-40 | AGA CGG ACA GGG GAG CC | 1/64 |
| TRBV20-1-41 | CCT AGC GGG GGG GC | 3/64 |
| TRBV20-1-42 | AGA GAC TCA GGG TTG AG | 2/64 |
| TRBV20-1-43 | AGA GAC GTG TGG GGG GG | 1/64 |
| TRBV20-1-44 | CCG GGA CAG GG | 1/64 |
Total RNA was isolated from the 1803Tu sample and reverse-transcribed into cDNA. TRBV20-1 sequences were amplified with the TRBV20-1-specific primer and sequenced. The D region sequences were compared. The TRBV20-1-13 sequence from the 1803FrTu sample has the same D region as the TRBV20-1 sequence from the 1803TIL sample, which is noted in bold.