American Association of Immunologists

Table II.

Serum Ab levels following intranasal immunization^a

Group	Time (mo)	Geometric Mean IgG Titers of Clade Ag									na CCR5 loop aa 168–185
Group	Time (mo)			B gp41 MN aa 661–675	A gp41 UG31 aa 652–665	C gp41 BR25 aa 651–665	D gp41 UG21 aa 643–657	B gp41 coil aa 578–592	B gp120 V3 aa 303–322	B rgp160Lai	na CCR5 loop aa 168–185
1	6	4,525	5,382	3,805	2,691	336	800	336	1,345
	12	3,805	3,200	2,691	1,345	336	336	566	1,903
2	6	3,676	4,850	4,222	4,222	400	696	459	<100
	12	1,832	3,200	2,111	1,838	200	132	400	<100
3	6	2,786	2,786	3,676	1,600	264	528	606	115
	12	2,111	1,600	1,600	919	174	100	400	159
4	6	<100	<100	<100	<100	<100	<100	<100	770
	12	<100	<100	<100	<100	<100	<100	<100	696
5	6	<100	<100	<100	<100	<100	<100	<100	<100
	12	<100	<100	<100	<100	<100	<100	<100	<100
mAb 2F5	Positive control^b	12,400	1,440	680	<100	<100	<100	57,600	<100

Group	Time (mo)	Geometric Mean IgG Titers of Clade Ag									na CCR5 loop aa 168–185
Group	Time (mo)			B gp41 MN aa 661–675	A gp41 UG31 aa 652–665	C gp41 BR25 aa 651–665	D gp41 UG21 aa 643–657	B gp41 coil aa 578–592	B gp120 V3 aa 303–322	B rgp160Lai	na CCR5 loop aa 168–185
1	6	4,525	5,382	3,805	2,691	336	800	336	1,345
	12	3,805	3,200	2,691	1,345	336	336	566	1,903
2	6	3,676	4,850	4,222	4,222	400	696	459	<100
	12	1,832	3,200	2,111	1,838	200	132	400	<100
3	6	2,786	2,786	3,676	1,600	264	528	606	115
	12	2,111	1,600	1,600	919	174	100	400	159
4	6	<100	<100	<100	<100	<100	<100	<100	770
	12	<100	<100	<100	<100	<100	<100	<100	696
5	6	<100	<100	<100	<100	<100	<100	<100	<100
	12	<100	<100	<100	<100	<100	<100	<100	<100
mAb 2F5	Positive control^b	12,400	1,440	680	<100	<100	<100	57,600	<100

IgG titers against HIV-1 gp41, gp120, rgp160, and CCR5 representing Ags 6 and 12 mo post-boost immunization. rgp160 = recombinant gp160, na, not applicable.

Positive IgG control 5 μg/ml (HRP-anti-IgG conjugate used). Statistical analysis using Mann-Whitney U nonparametric test were used to compare differences between groups and p = 0.05 was considered significant. No significant differences were seen when comparisons were performed between relevant groups.

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