American Association of Immunologists

Table IV.

Long-term HIV- and CCR5 Ag-specific IgA responses in lung and small intestinal washes 12 mos post-booster^a

Group	Sample^b	gp41 ELDKWAS IgA Peptide Titers/Clade													CCR5 Peptide	IgA Concn (μg/ml)
Group	Sample^b			gp41/A			gp41/B			gp41/C			gp41 Coil/B	rgp160	CCR5 Peptide	IgA Concn (μg/ml)
1	Lung	5	4	60	16	50	18	16	7	15	8	4	18 ± 6
	Intestine	46	46	80	90	50	80	16	12	30	30	20	57 ± 11
2	Lung	<2	<2	4	3	6	8	2	<2	2	2	<2	26 ± 9
	Intestine	12	12	20	28	16	16	<4	<4	8	8	<4	86 ± 19
3	Lung	<2	<2	10	2	10	4	<2	<2	<2	<2	<2	16,6 ± 9
	Intestine	4	4	20	28	<4	<4	<4	<4	4	8	<4	86 ± 27
4	Lung	<2	<2	<2	<2	<2	<2	<2	<2	<2	<2	<2	19,2 ± 8
	Intestine	<4	<4	<4	4	<4	<4	<4	<4	<4	<4	16	78 ± 11
5	Lung	2	<2	<2	<2	<2	<2	<2	<2	<2	<2	16	33 ± 16
	Intestine	<4	<4	<4	<4	<4	<4	<4	<4	<4	<4	<4	48 ± 8
Positive controls^c
mAb2F5		1,490		12,000		710		<100		61,000		<100
HIVIG		4,900		3,900		6,800		2,900		590,000		140
mAb2D7		nt		nt		nt		nt		<100		220
Negative control
Mouse sera		<50		<50		<50		<50		<50		<50

Group	Sample^b	gp41 ELDKWAS IgA Peptide Titers/Clade													CCR5 Peptide	IgA Concn (μg/ml)
Group	Sample^b			gp41/A			gp41/B			gp41/C			gp41 Coil/B	rgp160	CCR5 Peptide	IgA Concn (μg/ml)
1	Lung	5	4	60	16	50	18	16	7	15	8	4	18 ± 6
	Intestine	46	46	80	90	50	80	16	12	30	30	20	57 ± 11
2	Lung	<2	<2	4	3	6	8	2	<2	2	2	<2	26 ± 9
	Intestine	12	12	20	28	16	16	<4	<4	8	8	<4	86 ± 19
3	Lung	<2	<2	10	2	10	4	<2	<2	<2	<2	<2	16,6 ± 9
	Intestine	4	4	20	28	<4	<4	<4	<4	4	8	<4	86 ± 27
4	Lung	<2	<2	<2	<2	<2	<2	<2	<2	<2	<2	<2	19,2 ± 8
	Intestine	<4	<4	<4	4	<4	<4	<4	<4	<4	<4	16	78 ± 11
5	Lung	2	<2	<2	<2	<2	<2	<2	<2	<2	<2	16	33 ± 16
	Intestine	<4	<4	<4	<4	<4	<4	<4	<4	<4	<4	<4	48 ± 8
Positive controls^c
mAb2F5		1,490		12,000		710		<100		61,000		<100
HIVIG		4,900		3,900		6,800		2,900		590,000		140
mAb2D7		nt		nt		nt		nt		<100		220
Negative control
Mouse sera		<50		<50		<50		<50		<50		<50

Samples collected from the lung were tested at dilution 1/2–1/118 and intestinal washes at dilutions 1/4–1/256 at 2-fold serial serum dilutions. Sample IgA titer cut-off was calculated from mean absorbance values of negative control mice plus two SD. nt, not tested.

Two IgA pools per mouse group were prepared and tested.

Positive controls used were human IgG mAb and HIVIgG (HRP-anti-human IgG conjugate used) end-point titers are shown.

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