American Association of Immunologists

Table I.

Bacterial plasmids and strains used in this study

Strain	Plasmid	Phenotype^a	Reference
	pUW2138	Gent^r, OriT in pBluescript SK⁺	21
	pGB5P1	Kan^r, GFP in pBBRMCS-3	22
	pCW505	Kan^r, Gent^r, GFP in pBBRMCS-2	23
	pCW382-6	Kan^r, Cm^r, GFP in pBBRMCS-1	24
	pRK2013	ColE1 replicon, P-plasmid mobilization, Kan^r	25
	pKC109	Gent^r, OriT, 2.1 kb of adenylate cyclase toxin in Bluescript KS⁺	26
	pLMS023	859 bp of waaF, Gent^r, OriT in pBluescript KS⁺, amp^r, Kan^r	This study
BP338	pGB5P1	Tohama I, Wild-type LPS, Nal^r, Kan^r, GFP	27
BP536	pCW505	BP338, Wild type LPS, Nal^r, Kan^r, Strep^r, GFP	17
BP347	pCW505	BP338 with Tn5 insertion in bvg, avirulent, Nal^r, Kan^r, Gent^r, GFP	27
BPM3183	pCW505	BP338 with Tn5 lac insertion in adenylate cyclase toxin, Nal^r, Kan^r, Cm^r, GFP	28
MLT7	pCW505	BP338 with mini Tn5 insertion in LPS operon, Nal^r, Kan^r, Gent^r, GFP	19
WlbG	pCW382-6	BP536, ΔwlbG::Kan; Nal^r, Kan^r, Strep^r, Cm^r, GFP	17 18
WlbH	pCW382-6	BP536, ΔwlbH::Kan; Nal^r, Kan^r, Strep^r, Cm^r, GFP	17 18
WlbL	pCW382-6	BP536, ΔwlbL::Kan; Nal^r, Kan^r, Strep^r, Cm^r, GFP	17 18
WaaF	pCW382-6	BP338, waaF::pLMS023, Nal^r, Kan^r, Gent^r, Cm^r, GFP	This study
MLT7 cyc-	pCW382-6	MLT7, cyc::pKC109, Nal^r, Kan^r, Gent^r, Cm^r, GFP	This study
WlbG cyc-	pCW382-6	WlbG, cyc::pKC109, Nal^r, Kan^r, Gent^r, Strep^r, Cm^r, GFP	This study
WlbL cyc-	pCW382-6	WlbL, cyc::KC109, Nal^r, Kan^r, Gent^r, Strep^r, Cm^r, GFP	This study
Kpn		Klebsiella pneumoniae, human fecal isolate, susceptible to SP-A-mediated killing	29

Strain	Plasmid	Phenotype^a	Reference
	pUW2138	Gent^r, OriT in pBluescript SK⁺	21
	pGB5P1	Kan^r, GFP in pBBRMCS-3	22
	pCW505	Kan^r, Gent^r, GFP in pBBRMCS-2	23
	pCW382-6	Kan^r, Cm^r, GFP in pBBRMCS-1	24
	pRK2013	ColE1 replicon, P-plasmid mobilization, Kan^r	25
	pKC109	Gent^r, OriT, 2.1 kb of adenylate cyclase toxin in Bluescript KS⁺	26
	pLMS023	859 bp of waaF, Gent^r, OriT in pBluescript KS⁺, amp^r, Kan^r	This study
BP338	pGB5P1	Tohama I, Wild-type LPS, Nal^r, Kan^r, GFP	27
BP536	pCW505	BP338, Wild type LPS, Nal^r, Kan^r, Strep^r, GFP	17
BP347	pCW505	BP338 with Tn5 insertion in bvg, avirulent, Nal^r, Kan^r, Gent^r, GFP	27
BPM3183	pCW505	BP338 with Tn5 lac insertion in adenylate cyclase toxin, Nal^r, Kan^r, Cm^r, GFP	28
MLT7	pCW505	BP338 with mini Tn5 insertion in LPS operon, Nal^r, Kan^r, Gent^r, GFP	19
WlbG	pCW382-6	BP536, ΔwlbG::Kan; Nal^r, Kan^r, Strep^r, Cm^r, GFP	17 18
WlbH	pCW382-6	BP536, ΔwlbH::Kan; Nal^r, Kan^r, Strep^r, Cm^r, GFP	17 18
WlbL	pCW382-6	BP536, ΔwlbL::Kan; Nal^r, Kan^r, Strep^r, Cm^r, GFP	17 18
WaaF	pCW382-6	BP338, waaF::pLMS023, Nal^r, Kan^r, Gent^r, Cm^r, GFP	This study
MLT7 cyc-	pCW382-6	MLT7, cyc::pKC109, Nal^r, Kan^r, Gent^r, Cm^r, GFP	This study
WlbG cyc-	pCW382-6	WlbG, cyc::pKC109, Nal^r, Kan^r, Gent^r, Strep^r, Cm^r, GFP	This study
WlbL cyc-	pCW382-6	WlbL, cyc::KC109, Nal^r, Kan^r, Gent^r, Strep^r, Cm^r, GFP	This study
Kpn		Klebsiella pneumoniae, human fecal isolate, susceptible to SP-A-mediated killing	29

Gent^r, gentamicin resistant; OriT, origin of transfer P conjugation; Cm^r, chloramphenicol resistant; Kan^r, kanamycin resistant; Nal^r, nalidixic acid resistant; Strep^r, streptomycin resistant; amp^r, ampicillin resistant; cyc, adenylate cyclase toxin operon.

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