IL-27 elicits proliferation and IFN-γ production by myelin basic protein-specific CD4+ T cell line
| . | No Ag . | +Ag . | . | . | . | |||
|---|---|---|---|---|---|---|---|---|
| . | . | . | +β-Actin (50 ng/ml) . | +IL-27 (50 ng/ml) . | +IL-27 + Anti-IL-27 Ab . | |||
| IL-4 (pg/ml) | 12.7 ± 3.5 | 25 ± 7.8 | 23 ± 6 | 30 ± 5 | 27 ± 6 | |||
| IFN-γ (pg/ml) | 452 ± 25 | 520 ± 40 | 490 ± 50 | 1,130 ± 75 | 480 ± 60 | |||
| IL-10 (pg/ml) | 12 ± 5 | 12 ± 4 | 11 ± 3 | 12 ± 3 | 10 ± 4 | |||
| TNF-α | 295 ± 35 | 460 ± 80 | 450 ± 70 | 485 ± 50 | 480 ± 40 | |||
| Proliferation (cpm) | 880 ± 240 | 8,800 ± 930 | 8,950 ± 740 | 14,580 ± 720 | 8,300 ± 890 | |||
| . | No Ag . | +Ag . | . | . | . | |||
|---|---|---|---|---|---|---|---|---|
| . | . | . | +β-Actin (50 ng/ml) . | +IL-27 (50 ng/ml) . | +IL-27 + Anti-IL-27 Ab . | |||
| IL-4 (pg/ml) | 12.7 ± 3.5 | 25 ± 7.8 | 23 ± 6 | 30 ± 5 | 27 ± 6 | |||
| IFN-γ (pg/ml) | 452 ± 25 | 520 ± 40 | 490 ± 50 | 1,130 ± 75 | 480 ± 60 | |||
| IL-10 (pg/ml) | 12 ± 5 | 12 ± 4 | 11 ± 3 | 12 ± 3 | 10 ± 4 | |||
| TNF-α | 295 ± 35 | 460 ± 80 | 450 ± 70 | 485 ± 50 | 480 ± 40 | |||
| Proliferation (cpm) | 880 ± 240 | 8,800 ± 930 | 8,950 ± 740 | 14,580 ± 720 | 8,300 ± 890 | |||