α4 and α5 integrin chain expression as a function of TCRβ chain surface level in thymocytes from nkt/nkt or nkt/+ micea
| . | α4 . | . | . | . | α5 . | . | . | . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | Low/Int . | . | High . | . | Low/int . | . | High . | . | ||||||
| . | nkt/+ . | nkt/nkt . | nkt/+ . | nkt/nkt . | nkt/+ . | nkt/nkt . | nkt/+ . | nkt/nkt . | ||||||
| TCR−/low | 82.0 ± 3.6 | 74.8 ± 8.1 | 10.5 ± 4.2 | 11.7 ± 3.3 | 75.8 ± 2.6 | 71.9 ± 7.3 | 17.4 ± 1.6 | 16.8 ± 1.8 | ||||||
| TCRint | 88.6 ± 0.8 | 82.8 ± 4.7 | 8.0 ± 1.4 | 10.9 ± 1.4 | 61.6 ± 4.9 | 57.5 ± 5.7 | 36.3 ± 6.3 | 39.2 ± 5.1 | ||||||
| TCRhigh | 75.5 ± 2.9 | 68.3 ± 3.3 | 16.5 ± 4.7 | 22.2 ± 4.9 | 31.2 ± 3.9 | 26.1 ± 4.4 | 65.2 ± 5.0 | 70.8 ± 6.7 | ||||||
| . | α4 . | . | . | . | α5 . | . | . | . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | Low/Int . | . | High . | . | Low/int . | . | High . | . | ||||||
| . | nkt/+ . | nkt/nkt . | nkt/+ . | nkt/nkt . | nkt/+ . | nkt/nkt . | nkt/+ . | nkt/nkt . | ||||||
| TCR−/low | 82.0 ± 3.6 | 74.8 ± 8.1 | 10.5 ± 4.2 | 11.7 ± 3.3 | 75.8 ± 2.6 | 71.9 ± 7.3 | 17.4 ± 1.6 | 16.8 ± 1.8 | ||||||
| TCRint | 88.6 ± 0.8 | 82.8 ± 4.7 | 8.0 ± 1.4 | 10.9 ± 1.4 | 61.6 ± 4.9 | 57.5 ± 5.7 | 36.3 ± 6.3 | 39.2 ± 5.1 | ||||||
| TCRhigh | 75.5 ± 2.9 | 68.3 ± 3.3 | 16.5 ± 4.7 | 22.2 ± 4.9 | 31.2 ± 3.9 | 26.1 ± 4.4 | 65.2 ± 5.0 | 70.8 ± 6.7 | ||||||
Thymocytes from nkt/nkt and nkt/+ littermates were stained with anti-TCRβ chain and anti-CD49d or anti-CD49e and analyzed by flow cytometry. Values represent the mean percentages of cells expressing low/intermediate or high levels of α4 or α5 integrin chains ± SD (n = 4) within electonically gated populations expressing different levels of TCR. The experiment was performed three times with similar results.