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Table I.

Cytokine secretion by primary endometrial epithelial cells treated with the TLR3 agonist poly(I:C)a

IFN-γTNF-αIL-4IL-6IL-10GM-CSFIL-1βIL-12IL-17G-CSFCCL2CCL4
2739 Apical             
 No PAMP 47 48 b 580 b 1409 13 
 Poly(I:C) 51 276 b 1195 b 2027 240 
2749 Apical             
 No PAMP 47 157 b 554 b 1759 38 
 Poly(I:C) 55 522 b 989 b 2796 351 
2739 Basolateral             
 No PAMP 92 900 127 387 677 
 Poly(I:C) 105 56 4880 412 1315 1766 63 
2749 Basolateral             
 No PAMP 1557 419 305 885 19 
 Poly(I:C) 54 5348 572 890 1827 104 
IFN-γTNF-αIL-4IL-6IL-10GM-CSFIL-1βIL-12IL-17G-CSFCCL2CCL4
2739 Apical             
 No PAMP 47 48 b 580 b 1409 13 
 Poly(I:C) 51 276 b 1195 b 2027 240 
2749 Apical             
 No PAMP 47 157 b 554 b 1759 38 
 Poly(I:C) 55 522 b 989 b 2796 351 
2739 Basolateral             
 No PAMP 92 900 127 387 677 
 Poly(I:C) 105 56 4880 412 1315 1766 63 
2749 Basolateral             
 No PAMP 1557 419 305 885 19 
 Poly(I:C) 54 5348 572 890 1827 104 
a

Conditioned media were collected following 24-h PAMP stimulation and were analyzed for cytokine production secretion using the Luminex multiplex system. Cytokines are expressed in total picograms.

b

Above upper limit of detection, IL-6 (1888 pg/well), G-CSF (920 pg/well).

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