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Table I.

Surface receptor expression profiles of CD4+/Vβ8+ T cellsa

0 hb4 hb24 hb48 hb
Ly-49A+++++++
APCDdDdDbDbDdDdDbDbDdDdDbDb
Activation profilesc               
 CD25 10 ± 3 6 ± 2     9 ± 2d 25 ± 15 32 ± 16 30 ± 16 13 ± 6 15 ± 7 13 ± 4 14 ± 5 
 CD69 7 ± 2 7 ± 3     13 ± 2d 30 ± 15 38 ± 14 36 ± 17 14 ± 4 10 ± 5 16 ± 9 9 ± 6 
 CD62Llow 46 ± 22 37 ± 19     13 ± 5d 21 ± 10 24 ± 13 21 ± 11 10 ± 5 8 ± 6 9 ± 5 8 ± 6 
 CD40L 1 ± 0.5 1 ± 0.3 2 ± 1d 5 ± 2 4 ± 2 6 ± 3         
Activation profiles of T cells stimulated with Ag in the presence of 50 U/ml exogenous IL-2e               
 CD25 3 ± 1 3 ± 1     18 ± 10d 34 ± 18 37 ± 13 31 ± 9 18 ± 6d 41 ± 24 33 ± 14 33 ± 13 
 CD69 1 ± 1 1 ± 1     16 ± 8d 34 ± 9 43 ± 14 41 ± 12 14 ± 4d 27 ± 15 28 ± 10 28 ± 6 
Activation profiles of T cells following secondary challenge with Agf               
 CD25 45 ± 30 34 ± 17     33 ± 6d 53 ± 1 59 ± 3 53 ± 11 41 ± 26d 61 ± 36 56 ± 28 53 ± 31 
 CD69 12 ± 15 9 ± 11     36 ± 3d 46 ± 8 51 ± 5 48 ± 10 38 ± 17 44 ± 10 45 ± 12 46 ± 6 
 CD40L 3 ± 4 1 ± 1 5 ± 3d 24 ± 11 18 ± 8 22 ± 6         
0 hb4 hb24 hb48 hb
Ly-49A+++++++
APCDdDdDbDbDdDdDbDbDdDdDbDb
Activation profilesc               
 CD25 10 ± 3 6 ± 2     9 ± 2d 25 ± 15 32 ± 16 30 ± 16 13 ± 6 15 ± 7 13 ± 4 14 ± 5 
 CD69 7 ± 2 7 ± 3     13 ± 2d 30 ± 15 38 ± 14 36 ± 17 14 ± 4 10 ± 5 16 ± 9 9 ± 6 
 CD62Llow 46 ± 22 37 ± 19     13 ± 5d 21 ± 10 24 ± 13 21 ± 11 10 ± 5 8 ± 6 9 ± 5 8 ± 6 
 CD40L 1 ± 0.5 1 ± 0.3 2 ± 1d 5 ± 2 4 ± 2 6 ± 3         
Activation profiles of T cells stimulated with Ag in the presence of 50 U/ml exogenous IL-2e               
 CD25 3 ± 1 3 ± 1     18 ± 10d 34 ± 18 37 ± 13 31 ± 9 18 ± 6d 41 ± 24 33 ± 14 33 ± 13 
 CD69 1 ± 1 1 ± 1     16 ± 8d 34 ± 9 43 ± 14 41 ± 12 14 ± 4d 27 ± 15 28 ± 10 28 ± 6 
Activation profiles of T cells following secondary challenge with Agf               
 CD25 45 ± 30 34 ± 17     33 ± 6d 53 ± 1 59 ± 3 53 ± 11 41 ± 26d 61 ± 36 56 ± 28 53 ± 31 
 CD69 12 ± 15 9 ± 11     36 ± 3d 46 ± 8 51 ± 5 48 ± 10 38 ± 17 44 ± 10 45 ± 12 46 ± 6 
 CD40L 3 ± 4 1 ± 1 5 ± 3d 24 ± 11 18 ± 8 22 ± 6         
a

Purified Ly-49A/TCR-SFE (Ly-49A+) or TCR-SFE (Ly-49A) T cells were stimulated with SFE peptide and Dd or Db APC.

b

Time in culture. Analyses performed on freshly isolated T cells (only) are designated “0 h” except in the bottom section of profiles where “0 h“ indicates analyses performed on T cells following primary stimulation with Db APC for 72 h with a subsequent 5 day rest period in 20 U/ml rhIL-2.

c

Data are mean percent positive (±SD) of CD4+/Vβ8+ T cells obtained from at least five independent experiments. The percentage of CD4+/Vβ8+ cells expressing low levels of CD62L are indicated as CD62Llow (as in Fig. 2 C).

d

Mean values are significantly different compared to Ly-49A T cells and parallel control conditions using the Student t test (p < 0.05).

e

Cells were cultured as in the top section of profiles with the addition of 50 U/ml rhIL-2. Data are from at least three independent experiments.

f

Purified Ly-49A/TCR-SFE (Ly-49A+) or TCR-SFE (Ly-49A) T cells were cultured with SFE peptide and Db APC (primary stimulation) for 72 h, and then allowed to rest for 5 days in 20 U/ml rhIL-2 prior to secondary Ag challenge. Secondary stimulation was performed as outlined in the top section of profiles. Data are from at least three independent experiments.

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