Surface receptor expression profiles of CD4+/Vβ8+ T cellsa
| . | 0 hb . | . | 4 hb . | . | . | . | 24 hb . | . | . | . | 48 hb . | . | . | . | ||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Ly-49A . | + . | − . | + . | − . | + . | − . | + . | − . | + . | − . | + . | − . | + . | − . | ||||||||||||||
| APC . | . | . | Dd . | Dd . | Db . | Db . | Dd . | Dd . | Db . | Db . | Dd . | Dd . | Db . | Db . | ||||||||||||||
| Activation profilesc | ||||||||||||||||||||||||||||
| CD25 | 10 ± 3 | 6 ± 2 | 9 ± 2d | 25 ± 15 | 32 ± 16 | 30 ± 16 | 13 ± 6 | 15 ± 7 | 13 ± 4 | 14 ± 5 | ||||||||||||||||||
| CD69 | 7 ± 2 | 7 ± 3 | 13 ± 2d | 30 ± 15 | 38 ± 14 | 36 ± 17 | 14 ± 4 | 10 ± 5 | 16 ± 9 | 9 ± 6 | ||||||||||||||||||
| CD62Llow | 46 ± 22 | 37 ± 19 | 13 ± 5d | 21 ± 10 | 24 ± 13 | 21 ± 11 | 10 ± 5 | 8 ± 6 | 9 ± 5 | 8 ± 6 | ||||||||||||||||||
| CD40L | 1 ± 0.5 | 1 ± 0.3 | 2 ± 1d | 5 ± 2 | 4 ± 2 | 6 ± 3 | ||||||||||||||||||||||
| Activation profiles of T cells stimulated with Ag in the presence of 50 U/ml exogenous IL-2e | ||||||||||||||||||||||||||||
| CD25 | 3 ± 1 | 3 ± 1 | 18 ± 10d | 34 ± 18 | 37 ± 13 | 31 ± 9 | 18 ± 6d | 41 ± 24 | 33 ± 14 | 33 ± 13 | ||||||||||||||||||
| CD69 | 1 ± 1 | 1 ± 1 | 16 ± 8d | 34 ± 9 | 43 ± 14 | 41 ± 12 | 14 ± 4d | 27 ± 15 | 28 ± 10 | 28 ± 6 | ||||||||||||||||||
| Activation profiles of T cells following secondary challenge with Agf | ||||||||||||||||||||||||||||
| CD25 | 45 ± 30 | 34 ± 17 | 33 ± 6d | 53 ± 1 | 59 ± 3 | 53 ± 11 | 41 ± 26d | 61 ± 36 | 56 ± 28 | 53 ± 31 | ||||||||||||||||||
| CD69 | 12 ± 15 | 9 ± 11 | 36 ± 3d | 46 ± 8 | 51 ± 5 | 48 ± 10 | 38 ± 17 | 44 ± 10 | 45 ± 12 | 46 ± 6 | ||||||||||||||||||
| CD40L | 3 ± 4 | 1 ± 1 | 5 ± 3d | 24 ± 11 | 18 ± 8 | 22 ± 6 | ||||||||||||||||||||||
| . | 0 hb . | . | 4 hb . | . | . | . | 24 hb . | . | . | . | 48 hb . | . | . | . | ||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Ly-49A . | + . | − . | + . | − . | + . | − . | + . | − . | + . | − . | + . | − . | + . | − . | ||||||||||||||
| APC . | . | . | Dd . | Dd . | Db . | Db . | Dd . | Dd . | Db . | Db . | Dd . | Dd . | Db . | Db . | ||||||||||||||
| Activation profilesc | ||||||||||||||||||||||||||||
| CD25 | 10 ± 3 | 6 ± 2 | 9 ± 2d | 25 ± 15 | 32 ± 16 | 30 ± 16 | 13 ± 6 | 15 ± 7 | 13 ± 4 | 14 ± 5 | ||||||||||||||||||
| CD69 | 7 ± 2 | 7 ± 3 | 13 ± 2d | 30 ± 15 | 38 ± 14 | 36 ± 17 | 14 ± 4 | 10 ± 5 | 16 ± 9 | 9 ± 6 | ||||||||||||||||||
| CD62Llow | 46 ± 22 | 37 ± 19 | 13 ± 5d | 21 ± 10 | 24 ± 13 | 21 ± 11 | 10 ± 5 | 8 ± 6 | 9 ± 5 | 8 ± 6 | ||||||||||||||||||
| CD40L | 1 ± 0.5 | 1 ± 0.3 | 2 ± 1d | 5 ± 2 | 4 ± 2 | 6 ± 3 | ||||||||||||||||||||||
| Activation profiles of T cells stimulated with Ag in the presence of 50 U/ml exogenous IL-2e | ||||||||||||||||||||||||||||
| CD25 | 3 ± 1 | 3 ± 1 | 18 ± 10d | 34 ± 18 | 37 ± 13 | 31 ± 9 | 18 ± 6d | 41 ± 24 | 33 ± 14 | 33 ± 13 | ||||||||||||||||||
| CD69 | 1 ± 1 | 1 ± 1 | 16 ± 8d | 34 ± 9 | 43 ± 14 | 41 ± 12 | 14 ± 4d | 27 ± 15 | 28 ± 10 | 28 ± 6 | ||||||||||||||||||
| Activation profiles of T cells following secondary challenge with Agf | ||||||||||||||||||||||||||||
| CD25 | 45 ± 30 | 34 ± 17 | 33 ± 6d | 53 ± 1 | 59 ± 3 | 53 ± 11 | 41 ± 26d | 61 ± 36 | 56 ± 28 | 53 ± 31 | ||||||||||||||||||
| CD69 | 12 ± 15 | 9 ± 11 | 36 ± 3d | 46 ± 8 | 51 ± 5 | 48 ± 10 | 38 ± 17 | 44 ± 10 | 45 ± 12 | 46 ± 6 | ||||||||||||||||||
| CD40L | 3 ± 4 | 1 ± 1 | 5 ± 3d | 24 ± 11 | 18 ± 8 | 22 ± 6 | ||||||||||||||||||||||
Purified Ly-49A/TCR-SFE (Ly-49A+) or TCR-SFE (Ly-49A−) T cells were stimulated with SFE peptide and Dd or Db APC.
Time in culture. Analyses performed on freshly isolated T cells (only) are designated “0 h” except in the bottom section of profiles where “0 h“ indicates analyses performed on T cells following primary stimulation with Db APC for 72 h with a subsequent 5 day rest period in 20 U/ml rhIL-2.
Data are mean percent positive (±SD) of CD4+/Vβ8+ T cells obtained from at least five independent experiments. The percentage of CD4+/Vβ8+ cells expressing low levels of CD62L are indicated as CD62Llow (as in Fig. 2 C).
Mean values are significantly different compared to Ly-49A− T cells and parallel control conditions using the Student t test (p < 0.05).
Cells were cultured as in the top section of profiles with the addition of 50 U/ml rhIL-2. Data are from at least three independent experiments.
Purified Ly-49A/TCR-SFE (Ly-49A+) or TCR-SFE (Ly-49A−) T cells were cultured with SFE peptide and Db APC (primary stimulation) for 72 h, and then allowed to rest for 5 days in 20 U/ml rhIL-2 prior to secondary Ag challenge. Secondary stimulation was performed as outlined in the top section of profiles. Data are from at least three independent experiments.