American Association of Immunologists

Table I.

Tissue B cell depletion following CD20 mAb treatment^a

Tissue	B Subset^a	Control mAb^b	MB20–11 mAb	% Depletion
Bone marrow:	Pro/pre	2.7 ± 0.3 (17)	3.2 ± 0.5 (20)	0
	Immature	1.9 ± 0.2 (17)	1.6 ± 0.2 (20)	16
	Mature	2.6 ± 0.3 (17)	0.06 ± 0.01 (20)	98^d
Blood:	B220⁺	4.6 ± 0.4 (9)	0.08 ± 0.02 (12)	98^d
Spleen:	Mature	32.0 ± 3.4 (6)	0.7 ± 0.1 (6)	98^d
	T1	4.9 ± 1.0 (6)	0.62 ± 0.09 (6)	87^d
	T2	2.5 ± 0.7 (6)	0.01 ± 0.01 (6)	>99^d
	Marginal zone	1.6 ± 0.5 (6)	0.01 ± 0.01 (6)	>99^d
	B1a	1.3 ± 0.4 (3)	0.53 ± 0.08 (3)	60
	CD21⁻CD24⁻B220⁺	2.0 ± 0.4 (6)	1.4 ± 0.2 (6)	32
Peripheral LN:	B220⁺	1.1 ± 0.1 (16)	0.09 ± 0.02 (16)	95^d
Mesenteric LN:	B220⁺	3.1 ± 0.8 (4)	0.10 ± 0.03 (4)	97^d
Peyer’s patch:	B220⁺	0.7 ± 0.2 (4)	0.07 ± 0.02 (4)	91^d
Peritoneum:	B220⁺	1.8 ± 0.3 (14)	0.75 ± 0.09 (15)	59^d
	B1a (day 7)	0.40 ± 0.07 (14)	0.27 ± 0.04 (15)	33
	B1a (day 28)	0.73 ± 0.12 (5)	0.19 ± 0.09 (4)	74^c
	Bla (day 7)	0.41 ± 0.10 (10)	0.29 ± 0.05 (10)	30
	B2 (day 7)	0.94 ± 0.23 (14)	0.21 ± 0.04 (15)	78^d
	B2 (day 28)	2.3 ± 0.3 (5)	0.26 ± 0.06 (4)	89^d

Tissue	B Subset^a	Control mAb^b	MB20–11 mAb	% Depletion
Bone marrow:	Pro/pre	2.7 ± 0.3 (17)	3.2 ± 0.5 (20)	0
	Immature	1.9 ± 0.2 (17)	1.6 ± 0.2 (20)	16
	Mature	2.6 ± 0.3 (17)	0.06 ± 0.01 (20)	98^d
Blood:	B220⁺	4.6 ± 0.4 (9)	0.08 ± 0.02 (12)	98^d
Spleen:	Mature	32.0 ± 3.4 (6)	0.7 ± 0.1 (6)	98^d
	T1	4.9 ± 1.0 (6)	0.62 ± 0.09 (6)	87^d
	T2	2.5 ± 0.7 (6)	0.01 ± 0.01 (6)	>99^d
	Marginal zone	1.6 ± 0.5 (6)	0.01 ± 0.01 (6)	>99^d
	B1a	1.3 ± 0.4 (3)	0.53 ± 0.08 (3)	60
	CD21⁻CD24⁻B220⁺	2.0 ± 0.4 (6)	1.4 ± 0.2 (6)	32
Peripheral LN:	B220⁺	1.1 ± 0.1 (16)	0.09 ± 0.02 (16)	95^d
Mesenteric LN:	B220⁺	3.1 ± 0.8 (4)	0.10 ± 0.03 (4)	97^d
Peyer’s patch:	B220⁺	0.7 ± 0.2 (4)	0.07 ± 0.02 (4)	91^d
Peritoneum:	B220⁺	1.8 ± 0.3 (14)	0.75 ± 0.09 (15)	59^d
	B1a (day 7)	0.40 ± 0.07 (14)	0.27 ± 0.04 (15)	33
	B1a (day 28)	0.73 ± 0.12 (5)	0.19 ± 0.09 (4)	74^c
	Bla (day 7)	0.41 ± 0.10 (10)	0.29 ± 0.05 (10)	30
	B2 (day 7)	0.94 ± 0.23 (14)	0.21 ± 0.04 (15)	78^d
	B2 (day 28)	2.3 ± 0.3 (5)	0.26 ± 0.06 (4)	89^d

B cell subsets were: bone marrow pro/pre-B (IgM⁻ B220^low), immature B (IgM⁺B220^low); and mature B (IgM⁺B220^high); spleen mature (CD24⁺CD21⁺B220⁺), T1 (CD24^high CD21⁻B220⁺), T2 (CD24^highCD21⁺B220⁺), marginal zone (CD21^highCD1d⁺B220⁺), and Bla (CD5⁺B220^low); and peritoneal Bla (CD5⁺CD11b⁺IgM^highB220^low), B1b (CD5⁻CD11b⁺IgM^highB220^low), and B2 (CD5⁻IgM^lowB220^high). LN, lymph node.

Values (±SEM) indicate cell numbers present in mice 7 days after mAb treatment (250 μg). The number of mice examined is indicated in parentheses. Significant differences between means are indicated:

, p < 0.05;

, p < 0.01.

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