NCR expression in response to stimulation with TLR3 or TLR7/8 agonistsa
| . | NKp30 (%) . | NKp44 (%) . | NKp46 (%) . | NKG2D (%) . |
|---|---|---|---|---|
| PBS | 11.9 ± 4.5 | 5.6 ± 6.5 | 88.3 ± 18.2 | 4.0 ± 6.5 |
| Poly(I:C) (10 μg/ml) | 15.9 ± 8.5 | 8.0 ± 6.0 | 91.1 ± 14.2 | 6.4 ± 4.9 |
| R848 (3 μg/ml) | 13.2 ± 9.4 | 7.6 ± 2.9 | 85.9 ± 17.7 | 5.1 ± 2.7 |
| R848 (10 μg/ml) | 12.6 ± 7.2 | 7.1 ± 2.8 | 85.8 ± 18.6 | 3.5 ± 1.5 |
| . | NKp30 (%) . | NKp44 (%) . | NKp46 (%) . | NKG2D (%) . |
|---|---|---|---|---|
| PBS | 11.9 ± 4.5 | 5.6 ± 6.5 | 88.3 ± 18.2 | 4.0 ± 6.5 |
| Poly(I:C) (10 μg/ml) | 15.9 ± 8.5 | 8.0 ± 6.0 | 91.1 ± 14.2 | 6.4 ± 4.9 |
| R848 (3 μg/ml) | 13.2 ± 9.4 | 7.6 ± 2.9 | 85.9 ± 17.7 | 5.1 ± 2.7 |
| R848 (10 μg/ml) | 12.6 ± 7.2 | 7.1 ± 2.8 | 85.8 ± 18.6 | 3.5 ± 1.5 |
NK cells were purified and stimulated with poly(I:C) (10 μg/ml) or R848 (either 3 or 10 μg/ml) for 18 h at 37°C. Cells were stained for cell surface expression of NCRs and analyzed by flow cytometry. Results are expressed as the percentage of CD56+ cells that coexpress the indicated activatory receptor. A minimum of three donors were used (NKG2D experiment) and ranged from five to seven donors for the other activation antigens.