American Association of Immunologists

Table I.

Age-related differences in airway tissue eosinophilia and goblet cell hyperplasia after primary (1°) and secondary (2°) RSV infection^a

1° Infection	Sham	uvRSV	RSV	uvRSV	RSV
2° Infection				RSV	RSV
Tissue eosinophils (MBP-positive cells/mm BM)
Age at 1° infection
<1 wk	3.5 ± 0.8	3.3 ± 0.9	6.8 ± 1.0^b	2.2 ± 0.8	15.6 ± 3.8^c
3 wk	0.7 ± 0.3	0.5 ± 0.3	2.1 ± 0.9	1.5 ± 0.6	0.5 ± 0.1
6 wk	0.6 ± 0.3		1.6 ± 0.4
8 wk	0.6 ± 0.2		1.3 ± 0.4
Goblet cells (PAS-stained cells/mm BM)
Age at 1° infection
<1 wk	4.5 ± 1.2	5.0 ± 0.9	21.6 ± 3.0^b	9.8 ± 3.3^b	125.3 ± 15.8^c
3 wk	1.3 ± 0.2	1.8 ± 0.5	13.3 ± 2.5^b	12.3 ± 1.7^b	10.0 ± 2.3^c
6 wk	1.1 ± 0.2		11.0 ± 2.4^b
8 wk	1.0 ± 0.3		10.4 ± 2.3^b

1° Infection	Sham	uvRSV	RSV	uvRSV	RSV
2° Infection				RSV	RSV
Tissue eosinophils (MBP-positive cells/mm BM)
Age at 1° infection
<1 wk	3.5 ± 0.8	3.3 ± 0.9	6.8 ± 1.0^b	2.2 ± 0.8	15.6 ± 3.8^c
3 wk	0.7 ± 0.3	0.5 ± 0.3	2.1 ± 0.9	1.5 ± 0.6	0.5 ± 0.1
6 wk	0.6 ± 0.3		1.6 ± 0.4
8 wk	0.6 ± 0.2		1.3 ± 0.4
Goblet cells (PAS-stained cells/mm BM)
Age at 1° infection
<1 wk	4.5 ± 1.2	5.0 ± 0.9	21.6 ± 3.0^b	9.8 ± 3.3^b	125.3 ± 15.8^c
3 wk	1.3 ± 0.2	1.8 ± 0.5	13.3 ± 2.5^b	12.3 ± 1.7^b	10.0 ± 2.3^c
6 wk	1.1 ± 0.2		11.0 ± 2.4^b
8 wk	1.0 ± 0.3		10.4 ± 2.3^b

Data are expressed as the mean number of cells (±SEM) detected in central airways. Data are normalized to the length of airway epithelium BM.

Significant difference compared with age-matched, sham-inoculated, control group (p < 0.05).

Significant difference compared with 1° RSV in mice infected at <1 wk of age (p < 0.05).