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Table II.

Surface phenotype of macrophages treated with TNFα

Surface MarkerFraction Increase of MFI = MFI TNFα Treateda : MFI Untreated
Expt. 1Expt. 2Expt. 3Ave ± SD
F4/80 0.9 0.92 0.85 0.89 ± 0.04 
Gated on F4/80+ macrophages     
 CD11c 2.01 1.86 1.8 1.89 ± 0.11 
 CD11b 1.02 0.84 0.6 0.82 ± 0.21 
 CD40 2.22 2.47 2.3 2.33 ± 0.13 
 CD80 1.23 1.09 1.04 1.12 ± 0.10 
 CD86 1.7 1.42 1.38 1.5 ± 0.17 
 I-Ab 1.43 1.13 0.96 1.17 ± 0.24 
 CD14 3.04 1.39 2.24 2.22 ± 0.83 
 ICAM-1 7.97 2.73 5.38 5.36 ± 2.62 
Surface MarkerFraction Increase of MFI = MFI TNFα Treateda : MFI Untreated
Expt. 1Expt. 2Expt. 3Ave ± SD
F4/80 0.9 0.92 0.85 0.89 ± 0.04 
Gated on F4/80+ macrophages     
 CD11c 2.01 1.86 1.8 1.89 ± 0.11 
 CD11b 1.02 0.84 0.6 0.82 ± 0.21 
 CD40 2.22 2.47 2.3 2.33 ± 0.13 
 CD80 1.23 1.09 1.04 1.12 ± 0.10 
 CD86 1.7 1.42 1.38 1.5 ± 0.17 
 I-Ab 1.43 1.13 0.96 1.17 ± 0.24 
 CD14 3.04 1.39 2.24 2.22 ± 0.83 
 ICAM-1 7.97 2.73 5.38 5.36 ± 2.62 
a

Thioglycolate-induced PECs were treated with 30 ng/ml TNFα for 16 h or medium alone and then stained for surface markers. Ave, Average.

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