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Table I.

Reduced frequency of recovering caprin-1-null clones

ConstructTarget CellIntegrations at:Clones Analyzed
  Allele 1 Allele 2 Rda  
pPNT2-puro +/+ 2 (7.3%) 26 28 
pPNT2-bsr +/− ND 130 130 
pPNT3-bsr +/+ 10 (31.3%) 22 32 
pPNT3-bsr +/− 15 (16.3%)b 3 (3.2%) 74 92 
ConstructTarget CellIntegrations at:Clones Analyzed
  Allele 1 Allele 2 Rda  
pPNT2-puro +/+ 2 (7.3%) 26 28 
pPNT2-bsr +/− ND 130 130 
pPNT3-bsr +/+ 10 (31.3%) 22 32 
pPNT3-bsr +/− 15 (16.3%)b 3 (3.2%) 74 92 
a

Random integrations.

b

Replaces pPNT2-puro cassette.

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