American Association of Immunologists

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Table I.

Reduced frequency of recovering caprin-1-null clones

Construct	Target Cell	Integrations at:					Clones Analyzed
Construct	Target Cell			Allele 1	Allele 2	Rd^a	Clones Analyzed
pPNT2-puro	+/+	2 (7.3%)	0	26	28
pPNT2-bsr	+/−	ND	0	130	130
pPNT3-bsr	+/+	10 (31.3%)	0	22	32
pPNT3-bsr	+/−	15 (16.3%)^b	3 (3.2%)	74	92

Construct	Target Cell	Integrations at:					Clones Analyzed
Construct	Target Cell			Allele 1	Allele 2	Rd^a	Clones Analyzed
pPNT2-puro	+/+	2 (7.3%)	0	26	28
pPNT2-bsr	+/−	ND	0	130	130
pPNT3-bsr	+/+	10 (31.3%)	0	22	32
pPNT3-bsr	+/−	15 (16.3%)^b	3 (3.2%)	74	92

a

Random integrations.

b

Replaces pPNT2-puro cassette.