Depletion of CD4 TR cells facilitates priming of CD8 T cell responses
| B6 Micea . | Vaccinatedb . | Anti-CD4 Ab YTS 191.1-Treatedc . | Tet+ CD8 T Cells/Spleen (×104)d . |
|---|---|---|---|
| wt | − | − | <0.1 |
| wt | − | + | <0.1 |
| wt | + | − | 21.1 ± 1.9 |
| wt | − | + | 41.4 ± 5.9 |
| Aα−/− | − | − | <0.1 |
| Aα−/− | − | + | <0.1 |
| Aα−/− | + | − | 10.2 ± 0.4 |
| Aα−/− | + | + | 22.8 ± 0.9 |
| B6 Micea . | Vaccinatedb . | Anti-CD4 Ab YTS 191.1-Treatedc . | Tet+ CD8 T Cells/Spleen (×104)d . |
|---|---|---|---|
| wt | − | − | <0.1 |
| wt | − | + | <0.1 |
| wt | + | − | 21.1 ± 1.9 |
| wt | − | + | 41.4 ± 5.9 |
| Aα−/− | − | − | <0.1 |
| Aα−/− | − | + | <0.1 |
| Aα−/− | + | − | 10.2 ± 0.4 |
| Aα−/− | + | + | 22.8 ± 0.9 |
Sex- and age-matched wt or CD4 T cell-deficient (Aα−/−) B6 mice were used.
Mice were vaccinated once i.m. with 20 μg of HBsAg particles mixed with 20 μg of ODNs (as described in Materials and Methods).
Mice were depleted of CD4 T cells by repeated i.p. injections of anti-CD4 Ab (as described in Materials and Methods).
Mean numbers of tetramer+ (Tet+) CD8 T cells (± SEM) per spleen of five mice per group.