Oligonucleotide primers and probes used for real-time PCR
| Target Gene . | Typea . | Primer or Probe (5′–3′) . | Description (mer) . |
|---|---|---|---|
| mTREM-1 | F | CCAGAAGGCTTGGCAGAGACT | 22 |
| B | ACTTCCCCATGTGGACTTCACT | 22 | |
| mGAPDH | F | TGCAGTGGCAAAGTGGAGATT | 21 |
| B | ATTTGCCGTGAGTGGAGTCAT | 21 | |
| hTREM-1 | F | GCCTTGTGCCCACTCTATACCA | 22 |
| B | TGGAGACATCGGCAGTTGAC | 20 | |
| P | (FAM)CAGAACTGTGACCCAAGCTCCACCCA(TAMRA) | 26 | |
| hsTREM-1 | F | CCTCCCAAGGAGCCTCACA | 19 |
| B | ACACCGGAACCCCTTGGT | 18 | |
| P | (FAM)CTGTTCGATCGCATCCGCTTGGT(TAMRA) | 23 |
| Target Gene . | Typea . | Primer or Probe (5′–3′) . | Description (mer) . |
|---|---|---|---|
| mTREM-1 | F | CCAGAAGGCTTGGCAGAGACT | 22 |
| B | ACTTCCCCATGTGGACTTCACT | 22 | |
| mGAPDH | F | TGCAGTGGCAAAGTGGAGATT | 21 |
| B | ATTTGCCGTGAGTGGAGTCAT | 21 | |
| hTREM-1 | F | GCCTTGTGCCCACTCTATACCA | 22 |
| B | TGGAGACATCGGCAGTTGAC | 20 | |
| P | (FAM)CAGAACTGTGACCCAAGCTCCACCCA(TAMRA) | 26 | |
| hsTREM-1 | F | CCTCCCAAGGAGCCTCACA | 19 |
| B | ACACCGGAACCCCTTGGT | 18 | |
| P | (FAM)CTGTTCGATCGCATCCGCTTGGT(TAMRA) | 23 |
F, forward primer; B, backward primer; and P, TaqMan Probe.