Spleen cell responses from mice vaccinated with LiSIR2+/− mutants but not challenged with the WT parasitesa
| . | Con A . | . | . | SLA . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| . | IFN-γ . | IL-10 . | Ratio . | IFN-γ . | IL-10 . | Ratio . | ||||
| Uninfected | 10.88 ± 0.58 | 2.36 ± 0.57 | 4.61 | 313.40 ± 106.49 | 55.65 ± 6.91 | 5.63 | ||||
| Vaccinated and infected | 12.82 ± 0.56 | 2.19 ± 0.09 | 5.85 | 1250.80 ± 186.65 | 64.6 ± 4.78 | 19.36 | ||||
| Infected | 8.03 ± 1.01 | 3.63 ± 0.38 | 2.21 | 153.50 ± 27.99 | 155.25 ± 40.34 | 0.99 | ||||
| Vaccinated but not infected | 9.89 ± 0.23 | 2.15 ± 0.21 | 4.6 | 307.7 ± 56.0 | 67.53 ± 14.99 | 4.56 | ||||
| . | Con A . | . | . | SLA . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| . | IFN-γ . | IL-10 . | Ratio . | IFN-γ . | IL-10 . | Ratio . | ||||
| Uninfected | 10.88 ± 0.58 | 2.36 ± 0.57 | 4.61 | 313.40 ± 106.49 | 55.65 ± 6.91 | 5.63 | ||||
| Vaccinated and infected | 12.82 ± 0.56 | 2.19 ± 0.09 | 5.85 | 1250.80 ± 186.65 | 64.6 ± 4.78 | 19.36 | ||||
| Infected | 8.03 ± 1.01 | 3.63 ± 0.38 | 2.21 | 153.50 ± 27.99 | 155.25 ± 40.34 | 0.99 | ||||
| Vaccinated but not infected | 9.89 ± 0.23 | 2.15 ± 0.21 | 4.6 | 307.7 ± 56.0 | 67.53 ± 14.99 | 4.56 | ||||
Age-matched naive and 6-wk LiSIR2+/− mutant-infected mice were challenged or not with WT virulent clone (×108) and sacrificed after 14 days postinfection. Cells were stimulated with SLA (50 μg/ml) or ConA (6 μg/ml) for 72 h and the levels of secreted IFN-γ and IL-10 were measured by ELISA as described in Materials and Methods. The IFN-γ and IL-10 values represented under Con A stimulation are in ng/ml and those represented under SLA stimulation are in pg/ml. The corresponding IFN-γ /IL-10 ratio is shown for each group of mice. The data represent means ± SD from triplicate cultures of four animals analyzed individually and are representative of three independent experiments.