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Table I.

After a bout of acute EAE caused by p178, BV18 and BV19 TCRs can be found in the spleen at all stages of the development of EAE

EAEa Phases (Day after Immunization)Vβ-Jβ (Base)b RearrangementNo. Positive Spleens/No. Total SpleensPresence in the CNS
Remission (day 20) Vβ19-Jβ1.2 (127) 1/4 − 
 Vβ18-Jβ1.2 (132) 2/4 − 
First relapse (days 25–26) Vβ19-Jβ1.2 (124) 1/4 − 
 Vβ19-Jβ1.2 (121) 1/4 − 
 Vβ18-Jβ1.2 (132) 1/4 − 
Late/chronic (day 45) Vβ19-Jβ1.2 (124) 2/4 − 
 Vβ18-Jβ1.2 (132) 1/4 − 
EAEa Phases (Day after Immunization)Vβ-Jβ (Base)b RearrangementNo. Positive Spleens/No. Total SpleensPresence in the CNS
Remission (day 20) Vβ19-Jβ1.2 (127) 1/4 − 
 Vβ18-Jβ1.2 (132) 2/4 − 
First relapse (days 25–26) Vβ19-Jβ1.2 (124) 1/4 − 
 Vβ19-Jβ1.2 (121) 1/4 − 
 Vβ18-Jβ1.2 (132) 1/4 − 
Late/chronic (day 45) Vβ19-Jβ1.2 (124) 2/4 − 
 Vβ18-Jβ1.2 (132) 1/4 − 
a

EAE was induced in SJL mice by immunization with 100 μg/mouse of p178 resuspended in PBS and emulsified 1/1 with enriched CFA. Immunization was followed by injection of pertussis toxin at days 0 and 3, and disease score was assessed according to the scale indicated in Materials and Methods.

b

Length of the rearrangement product obtained using the primers detailed in Ref. 28 .

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