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Table II.

Properties of fluorescein-binding scFvs

NameDissociation half-time at
pH 7.4, 25°CapH 5.4, 37°C
4M2.3 22 min <1 min 
4M3.12 4.0 h 4.8 min 
4M4.5 26 h 9.8 min 
4M5.3 5.7 days 1.9 h 
NameDissociation half-time at
pH 7.4, 25°CapH 5.4, 37°C
4M2.3 22 min <1 min 
4M3.12 4.0 h 4.8 min 
4M4.5 26 h 9.8 min 
4M5.3 5.7 days 1.9 h 
a

From Ref. 23 .

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