American Association of Immunologists

Table I.

Pervasive and stochastic expansion of nonclonotypic T cells involves many endogenous Vβ families^a

Vβ Family	OT-II B6							OT-II Sf
Vβ Family		Vα2 Expression in Each Vβ Family
	Vα2^high	Vα2^int	Vα2^neg	Total	Vα2^high	Vα2^int	Vα2^neg	Total^b
Vβ2	1.3	1.0	0.0	2.3	1.2	3.3	0.0	4.5
Vβ3	0.8	0.0	0.0	0.8	0.8	1.9	0.0	2.7
Vβ4	0.6	0.0	0.1	0.7	0.9	1.9	0.0	2.8
Vβ5.1/5.2	91.0	3.3	0.0	94.3	45.0	12.0	1.1	58.1
Vβ6	0.5	0.0	0.0	0.5	2.2	3.6	0.7	6.5
Vβ7	1.1	0.0	0.0	1.1	0.7	1.2	0.0	1.9
Vβ8.1	0.9	0.0	0.1	0.9	1.4	3.3	2.1	6.8
Vβ8.3	0.5	0.0	0.0	0.5	0.6	2.2	0.0	2.8
Vβ9	0.1	0.0	0.0	0.1	0.3	1.0	0.0	1.3
Vβ10	0.1	0.0	0.0	0.1	0.5	2.0	0.0	2.5
Vβ11	0.4	0.0	0.1	0.5	0.4	1.6	0.0	2.0
Vβ12	0.7	0.0	0.0	0.7	0.6	1.9	0.0	2.5
Vβ13	0.4	0.0	0.0	0.4	0.6	1.9	0.0	2.5
Vβ14	1.1	0.0	0.0	1.1	1.4	4.6	0.7	6.7
Total	99.5	4.3	0.3	104.0	56.6	42.4	4.6	103.6

Vβ Family	OT-II B6							OT-II Sf
Vβ Family		Vα2 Expression in Each Vβ Family
	Vα2^high	Vα2^int	Vα2^neg	Total	Vα2^high	Vα2^int	Vα2^neg	Total^b
Vβ2	1.3	1.0	0.0	2.3	1.2	3.3	0.0	4.5
Vβ3	0.8	0.0	0.0	0.8	0.8	1.9	0.0	2.7
Vβ4	0.6	0.0	0.1	0.7	0.9	1.9	0.0	2.8
Vβ5.1/5.2	91.0	3.3	0.0	94.3	45.0	12.0	1.1	58.1
Vβ6	0.5	0.0	0.0	0.5	2.2	3.6	0.7	6.5
Vβ7	1.1	0.0	0.0	1.1	0.7	1.2	0.0	1.9
Vβ8.1	0.9	0.0	0.1	0.9	1.4	3.3	2.1	6.8
Vβ8.3	0.5	0.0	0.0	0.5	0.6	2.2	0.0	2.8
Vβ9	0.1	0.0	0.0	0.1	0.3	1.0	0.0	1.3
Vβ10	0.1	0.0	0.0	0.1	0.5	2.0	0.0	2.5
Vβ11	0.4	0.0	0.1	0.5	0.4	1.6	0.0	2.0
Vβ12	0.7	0.0	0.0	0.7	0.6	1.9	0.0	2.5
Vβ13	0.4	0.0	0.0	0.4	0.6	1.9	0.0	2.5
Vβ14	1.1	0.0	0.0	1.1	1.4	4.6	0.7	6.7
Total	99.5	4.3	0.3	104.0	56.6	42.4	4.6	103.6

The data are derived from data obtained from flow cytometry staining using anti-CD4, anti-Vα2, and various anti-Vβ family mAb. Numbers represent the percentage of cells expressing the indicated phenotypes in the CD4⁺ T cell population. High, intermediate, and low expression levels of Vα2 are indicated as Vα2^high, Vα2^int, and Vα2^neg, respectively. Three mouse samples were studied and a representative one is shown.

Comparing the expression levels of each Vβ family member between OT-II B6 and OT-II Sf and between OT-II Sf with B6 (Ref. 21 and data not shown) indicated the stochastic nature of the expansion of the endogenous Vβ families.

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