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Table I.

Pervasive and stochastic expansion of nonclonotypic T cells involves many endogenous Vβ familiesa

Vβ FamilyOT-II B6OT-II Sf
Vα2 Expression in Each Vβ Family
Vα2highVα2intVα2negTotalVα2highVα2intVα2negTotalb
Vβ2 1.3 1.0 0.0 2.3 1.2 3.3 0.0 4.5 
Vβ3 0.8 0.0 0.0 0.8 0.8 1.9 0.0 2.7 
Vβ4 0.6 0.0 0.1 0.7 0.9 1.9 0.0 2.8 
Vβ5.1/5.2 91.0 3.3 0.0 94.3 45.0 12.0 1.1 58.1 
Vβ6 0.5 0.0 0.0 0.5 2.2 3.6 0.7 6.5 
Vβ7 1.1 0.0 0.0 1.1 0.7 1.2 0.0 1.9 
Vβ8.1 0.9 0.0 0.1 0.9 1.4 3.3 2.1 6.8 
Vβ8.3 0.5 0.0 0.0 0.5 0.6 2.2 0.0 2.8 
Vβ9 0.1 0.0 0.0 0.1 0.3 1.0 0.0 1.3 
Vβ10 0.1 0.0 0.0 0.1 0.5 2.0 0.0 2.5 
Vβ11 0.4 0.0 0.1 0.5 0.4 1.6 0.0 2.0 
Vβ12 0.7 0.0 0.0 0.7 0.6 1.9 0.0 2.5 
Vβ13 0.4 0.0 0.0 0.4 0.6 1.9 0.0 2.5 
Vβ14 1.1 0.0 0.0 1.1 1.4 4.6 0.7 6.7 
Total 99.5 4.3 0.3 104.0 56.6 42.4 4.6 103.6 
Vβ FamilyOT-II B6OT-II Sf
Vα2 Expression in Each Vβ Family
Vα2highVα2intVα2negTotalVα2highVα2intVα2negTotalb
Vβ2 1.3 1.0 0.0 2.3 1.2 3.3 0.0 4.5 
Vβ3 0.8 0.0 0.0 0.8 0.8 1.9 0.0 2.7 
Vβ4 0.6 0.0 0.1 0.7 0.9 1.9 0.0 2.8 
Vβ5.1/5.2 91.0 3.3 0.0 94.3 45.0 12.0 1.1 58.1 
Vβ6 0.5 0.0 0.0 0.5 2.2 3.6 0.7 6.5 
Vβ7 1.1 0.0 0.0 1.1 0.7 1.2 0.0 1.9 
Vβ8.1 0.9 0.0 0.1 0.9 1.4 3.3 2.1 6.8 
Vβ8.3 0.5 0.0 0.0 0.5 0.6 2.2 0.0 2.8 
Vβ9 0.1 0.0 0.0 0.1 0.3 1.0 0.0 1.3 
Vβ10 0.1 0.0 0.0 0.1 0.5 2.0 0.0 2.5 
Vβ11 0.4 0.0 0.1 0.5 0.4 1.6 0.0 2.0 
Vβ12 0.7 0.0 0.0 0.7 0.6 1.9 0.0 2.5 
Vβ13 0.4 0.0 0.0 0.4 0.6 1.9 0.0 2.5 
Vβ14 1.1 0.0 0.0 1.1 1.4 4.6 0.7 6.7 
Total 99.5 4.3 0.3 104.0 56.6 42.4 4.6 103.6 
a

The data are derived from data obtained from flow cytometry staining using anti-CD4, anti-Vα2, and various anti-Vβ family mAb. Numbers represent the percentage of cells expressing the indicated phenotypes in the CD4+ T cell population. High, intermediate, and low expression levels of Vα2 are indicated as Vα2high, Vα2int, and Vα2neg, respectively. Three mouse samples were studied and a representative one is shown.

b

Comparing the expression levels of each Vβ family member between OT-II B6 and OT-II Sf and between OT-II Sf with B6 (Ref. 21 and data not shown) indicated the stochastic nature of the expansion of the endogenous Vβ families.

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