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Table III.

Effects of anti-MIF Ab on particle transport by Caco-2 cells cocultured with Raji B cellsa

TreatmentNo.k (min−1)ymax − y0
MeanSDMeanSD
Expt. A      
 Raji 12 0.168(a) 0.144 4.24(a) 2.407 
 Raji + αMIF 12 0.061(b) 0.117 1.53(b) 2.585 
 None 12 0.003(b) 0.008 0.19(b) 0.419 
Expt. B      
 Raji 12 0.034(a) 0.023 1.934(a) 0.615 
 Raji + αMIF 0.010(b) 0.011 1.035(b) 1.165 
 None 10 0.003(b) 0.003 0.424(b) 0.402 
TreatmentNo.k (min−1)ymax − y0
MeanSDMeanSD
Expt. A      
 Raji 12 0.168(a) 0.144 4.24(a) 2.407 
 Raji + αMIF 12 0.061(b) 0.117 1.53(b) 2.585 
 None 12 0.003(b) 0.008 0.19(b) 0.419 
Expt. B      
 Raji 12 0.034(a) 0.023 1.934(a) 0.615 
 Raji + αMIF 0.010(b) 0.011 1.035(b) 1.165 
 None 10 0.003(b) 0.003 0.424(b) 0.402 
a

Mean ± SD of the maximum relative rate of fluorescence increase (k), and the difference between the logarithms of the final and initial fluorescence intensities (ymax − y0), of particles transported by Caco-2 cells cultured alone (None), or with Raji B cells either in the presence of 0.1 μg/ml (Expt. A) or 0.2 μg/ml (Expt. B) or absence (Raji) of anti-MIF Ab. Equal letters denote means that are not significantly different according to a Tukey test (99% confidence). Means indicated with “a” are significantly different from means indicated with “b” (No. = number of Transwells).

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