Spinal cord 1,25-(OH)2D3 in SHAM and OVX mice with placebo or E2 implants and fed 0 or 1 μg/day vitamin D3
| Surgerya . | Vitamin D3 (μg/day) . | E2 Implant (mg) . | Spinal Cord 1,25-(OH)2D3b (fmol/g) . |
|---|---|---|---|
| SHAM | 0 | 0 | 44 ± 24 |
| SHAM | 1 | 0 | 226 ± 293 |
| OVX | 0 | 0 | 59 ± 22 |
| OVX | 1 | 0 | 189 ± 64** |
| OVX | 0 | 0.1 | 29 ± 5 |
| OVX | 1 | 0.1 | 178 ± 92** |
| Surgerya . | Vitamin D3 (μg/day) . | E2 Implant (mg) . | Spinal Cord 1,25-(OH)2D3b (fmol/g) . |
|---|---|---|---|
| SHAM | 0 | 0 | 44 ± 24 |
| SHAM | 1 | 0 | 226 ± 293 |
| OVX | 0 | 0 | 59 ± 22 |
| OVX | 1 | 0 | 189 ± 64** |
| OVX | 0 | 0.1 | 29 ± 5 |
| OVX | 1 | 0.1 | 178 ± 92** |
Female B6 mice were fed diets with 0 or 1 μg/day vitamin D3 continuously beginning 1 wk before surgery. OVX or SHAM surgery was performed and a placebo or E2 pellet (0.1 mg; 60-day release) was implanted s.c. in each OVX mouse. Two weeks after surgery the mice were immunized with MOG35–55 peptide.
Spinal cord 1,25-(OH)2D3 were quantified at the end of the study. The data shown are the means ± SD (≥6–7 mice/group) from one experiment of three. **, p < 0.005 for comparisons between −D and +D mice within the same surgical and implant groups (Mann-Whitney test).