Dose response of PGE2 produced by normal human OB in the presence of MSUM or CPPD microcrystalsa
| . | PGE2 (ng/mg protein) . | p . |
|---|---|---|
| Control | 1.7 ± 0.5 (18) | |
| MSUM (mg/ml) | ||
| 0.01 | 8.6 ± 6.2 (5) | NS |
| 0.03 | 17.4 ± 13.0 (6) | NS |
| 0.1 | 14.8 ± 9.4 (11) | NS |
| 0.3 | 22.0 ± 12.4 (18) | 0.01 |
| 1.0 | 58.9 ± 27.0 (11) | 0.006 |
| 3.0 | 37.6 ± 24.6 (6) | NS |
| CPPD (mg/ml) | ||
| 0.01 | 13.6 ± 11.5 (5) | NS |
| 0.03 | 12.3 ± 7.8 (6) | NS |
| 0.1 | 21.1 ± 13.9 (6) | 0.05 |
| 0.3 | 25.4 ± 19.9 (11) | 0.05 |
| 1.0 | 24.3 ± 11.1 (18) | 0.007 |
| 3.0 | 10.4 ± 5.1 (6) | NS |
| . | PGE2 (ng/mg protein) . | p . |
|---|---|---|
| Control | 1.7 ± 0.5 (18) | |
| MSUM (mg/ml) | ||
| 0.01 | 8.6 ± 6.2 (5) | NS |
| 0.03 | 17.4 ± 13.0 (6) | NS |
| 0.1 | 14.8 ± 9.4 (11) | NS |
| 0.3 | 22.0 ± 12.4 (18) | 0.01 |
| 1.0 | 58.9 ± 27.0 (11) | 0.006 |
| 3.0 | 37.6 ± 24.6 (6) | NS |
| CPPD (mg/ml) | ||
| 0.01 | 13.6 ± 11.5 (5) | NS |
| 0.03 | 12.3 ± 7.8 (6) | NS |
| 0.1 | 21.1 ± 13.9 (6) | 0.05 |
| 0.3 | 25.4 ± 19.9 (11) | 0.05 |
| 1.0 | 24.3 ± 11.1 (18) | 0.007 |
| 3.0 | 10.4 ± 5.1 (6) | NS |
After 24 h incubation, PGE2 was measured in supernatants by EIA with AChE as tracer (see Materials and Methods). Results are expressed as means ± SEM. Statistical analysis used the Wilcoxon signed rank test; microcrystal data were compared with control data, and significance was set at 0.05. Numbers in parentheses, number of experiments tested.