| . | . | BM . | Spleen . | Lymph Nodes . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| . | Weeks Reconstituted . | WT . | C-chim . | UV-chim . | WT . | C-chim . | UV-chim . | WT . | C-chim . | UV-chim . |
| Total cells (×106) | 4 | 55.8 ± 1.9 | 55.3 ± 2.4 | 49.3 ± 1.7 | 103.2 ± 4.0 | 83.8 ± 18.6 | 106.5 ± 22.5 | — | — | — |
| 12 | 62.0 ± 11.8 | 71.7 ± 19.5 | 66.2 ± 6.4 | 74.5 ± 6.5 | 101.5 ± 4.5 | 77.5 ± 7.5 | — | — | — | |
| % CD19+B220+ | 12 | 25.0 ± 3.6 | 29.7 ± 2.3 | 29.7 ± 0.9 | 41.8 ± 5.0 | 59.2 ± 1.9 | 55.4 ± 2.5 | 39.5 ± 1.4 | 35.9 ± 2.2 | 36.8 ± 2.8 |
| % CD45.1 | 0.10 | 97.4 | 96.4 | 0.0 | 99.2 | 99.3 | 0.0 | 99.4 | 99.3 | |
| % CD3+CD4− | 12 | 2.9 ± 0.2 | 3.1 ± 0.1 | 3.2 ± 0.4 | 10.3 ± 0.8 | 7.7 ± 0.6 | 10.0 ± 1.4 | 28.5 ± 0.8 | 27.5 ± 1.0 | 27.2 ± 2.2 |
| % CD45.1 | 0.9 | 85.7 | 81.1 | 0.1 | 92.0 | 89.6 | 0.0 | 94.0 | 89.9 | |
| % CD3+CD4+ | 12 | 1.0 ± 0.1 | 1.2 ± 0.1 | 1.0 ± 0.1 | 12.2 ± 1.2 | 10.1 ± 0.6 | 13.3 ± 2.2 | 22.9 ± 1.2 | 31.8 ± 1.0 | 30.0 ± 1.6 |
| % CD45.1 | 0.04 | 83.1 | 76.7 | 0.0 | 93.7 | 90.0 | 0.0 | 95.8 | 92.9 | |
| % F4/80+CD11b+ | 12 | 8.9 ± 1.4 | 9.3 ± 0.8 | 10.8 ± 1.2 | 1.9 ± 0.3 | 3.2 ± 0.8 | 2.9 ± 0.3 | 0.9 ± 0.2 | 0.7 ± 0.2 | 0.8 ± 0.3 |
| % CD45.1 | 5.5 | 99.6 | 99.7 | 9.6 | 98.2 | 99.1 | 6.2 | 97.9 | 97.0 | |
| . | . | BM . | Spleen . | Lymph Nodes . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| . | Weeks Reconstituted . | WT . | C-chim . | UV-chim . | WT . | C-chim . | UV-chim . | WT . | C-chim . | UV-chim . |
| Total cells (×106) | 4 | 55.8 ± 1.9 | 55.3 ± 2.4 | 49.3 ± 1.7 | 103.2 ± 4.0 | 83.8 ± 18.6 | 106.5 ± 22.5 | — | — | — |
| 12 | 62.0 ± 11.8 | 71.7 ± 19.5 | 66.2 ± 6.4 | 74.5 ± 6.5 | 101.5 ± 4.5 | 77.5 ± 7.5 | — | — | — | |
| % CD19+B220+ | 12 | 25.0 ± 3.6 | 29.7 ± 2.3 | 29.7 ± 0.9 | 41.8 ± 5.0 | 59.2 ± 1.9 | 55.4 ± 2.5 | 39.5 ± 1.4 | 35.9 ± 2.2 | 36.8 ± 2.8 |
| % CD45.1 | 0.10 | 97.4 | 96.4 | 0.0 | 99.2 | 99.3 | 0.0 | 99.4 | 99.3 | |
| % CD3+CD4− | 12 | 2.9 ± 0.2 | 3.1 ± 0.1 | 3.2 ± 0.4 | 10.3 ± 0.8 | 7.7 ± 0.6 | 10.0 ± 1.4 | 28.5 ± 0.8 | 27.5 ± 1.0 | 27.2 ± 2.2 |
| % CD45.1 | 0.9 | 85.7 | 81.1 | 0.1 | 92.0 | 89.6 | 0.0 | 94.0 | 89.9 | |
| % CD3+CD4+ | 12 | 1.0 ± 0.1 | 1.2 ± 0.1 | 1.0 ± 0.1 | 12.2 ± 1.2 | 10.1 ± 0.6 | 13.3 ± 2.2 | 22.9 ± 1.2 | 31.8 ± 1.0 | 30.0 ± 1.6 |
| % CD45.1 | 0.04 | 83.1 | 76.7 | 0.0 | 93.7 | 90.0 | 0.0 | 95.8 | 92.9 | |
| % F4/80+CD11b+ | 12 | 8.9 ± 1.4 | 9.3 ± 0.8 | 10.8 ± 1.2 | 1.9 ± 0.3 | 3.2 ± 0.8 | 2.9 ± 0.3 | 0.9 ± 0.2 | 0.7 ± 0.2 | 0.8 ± 0.3 |
| % CD45.1 | 5.5 | 99.6 | 99.7 | 9.6 | 98.2 | 99.1 | 6.2 | 97.9 | 97.0 | |
Comparisons of cell yields and cell types from wild-type (WT; nonchimeric) mice are shown at 4 and 12 wk after injection of cells. Mean ± SEM for three individual mice.
C-chim, control-chimeric; UV-chim, UV-chimeric.